Basophils help establish protective immunity induced by irradiated larval vaccination for filariasis.
Basophils help establish protective immunity induced by irradiated larval vaccination for filariasis.
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DOI：
10.1016/j.vaccine.2013.06.010
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发表时间：
2013-08-12
期刊：
影响因子：
5.5
通讯作者：
Mitre E
中科院分区：
文献类型：
--
作者：
Torrero MN;Morris CP;Mitre BK;Hübner MP;Fox EM;Karasuyama H;Mitre E
嗜碱性细胞在对蠕虫的免疫应答中发挥着重要作用。在这项研究中，我们评估了嗜碱性细胞在疫苗介导的预防丝虫、导致象皮病和河盲症等疾病的组织侵袭性寄生线虫中的作用。用照射后的L3期幼虫免疫BALB/c小鼠，每周注射抗CD200R3抗体，去除嗜碱性粒细胞，观察小鼠的保护性免疫和免疫应答。接种疫苗方案后，攻击性感染前嗜碱性粒细胞的耗尽不会改变保护性免疫。相比之下，嗜碱性细胞在接种前开始耗尽，并在挑战感染后持续，显著削弱了接种疫苗所产生的保护作用。在嗜碱性粒细胞耗竭的小鼠中，疫苗诱导的对寄生虫抗原的细胞免疫反应显著减少，CD_4+T细胞产生IL-4、IL-5、IL-10和干扰素-γ显著减少。有趣的是，皮肤肥大细胞的数量在接种照射后的L3幼虫后显著增加，而在碱性粒细胞耗竭的小鼠中接种后没有变化。这些发现表明，嗜碱性粒细胞有助于建立负责辐射L3疫苗保护的免疫反应。
Basophils are increasingly recognized as playing important roles in the immune response towards helminths. In this study, we evaluated the role of basophils in vaccine-mediated protection against filariae, tissue-invasive parasitic nematodes responsible for diseases such as elephantiasis and river blindness. Protective immunity and immunological responses were assessed in BALB/c mice vaccinated with irradiated L3 stage larvae and depleted of basophils with weekly injections of anti-CD200R3 antibody. Depletion of basophils after administration of the vaccination regimen but before challenge infection did not alter protective immunity. In contrast, basophil depletion initiated prior to vaccination and continued after challenge infection significantly attenuated the protective effect conferred by vaccination. Vaccine-induced cellular immune responses to parasite antigen were substantially decreased in basophil-depleted mice, with significant decreases in CD4+ T-cell production of IL-4, IL-5, IL-10, and IFN-γ. Interestingly, skin mast cell numbers, which increased significantly after vaccination with irradiated L3 larvae, were unchanged after vaccination in basophil-depleted mice. These findings demonstrate that basophils help establish the immune responses responsible for irradiated L3 vaccine protection.