Coculture of Osteoblasts and Endothelial Cells: Optimization of Culture Medium and Cell Ratio
Coculture of Osteoblasts and Endothelial Cells: Optimization of Culture Medium and Cell Ratio
复制标题
成骨细胞和内皮细胞共培养：培养基和细胞比例的优化
DOI：
10.1089/ten.tec.2010.0215
复制
发表时间：
2011-03-01
影响因子：
3
通讯作者：
Jansen, John A.
中科院分区：
文献类型：
--
作者：
Ma, Jinling;van den Beucken, Jeroen J. J. P.;Jansen, John A.
骨组织工程血管化的策略依赖于最佳的培养条件。本研究的目的是确定最佳的细胞培养基和细胞比例的共培养的人骨髓基质细胞（HMSCs）和人脐静脉内皮细胞（HUVECs）的成骨和血管生成的结果参数后，二维和三维培养条件。在四种不同的培养基中进行培养：成骨细胞增殖培养基、成骨培养基（OM）、内皮培养基和后两种培养基的1：1混合物。仅在OM中观察到共培养物内的矿化。随后在OM中的实验表明，在HMSC/HUVEC比例为50：50的共培养物中，碱性磷酸酶活性、矿化和CD 31（+）染色最高。因此，本研究的结果表明，在OM中HMSC/HUVEC共培养比例为50：50是获得成骨和血管生成分化的最佳组合。
Vascularization strategies in cell-based bone tissue engineering depend on optimal culture conditions. The present study aimed to determine optimal cell culture medium and cell ratio for cocultures of human marrow stromal cells (HMSCs) and human umbilical vein endothelial cells (HUVECs) in view of both osteogenic and angiogenic outcome parameters upon two-dimensional and three-dimensional culture conditions. Cultures were performed in four different media: osteoblastic cell proliferation medium, osteogenic medium (OM), endothelial medium, and a 1:1 mixture of the latter two media. Mineralization within the cocultures was observed only in OM. Subsequent experiments in OM showed that alkaline phosphatase activity, mineralization, and CD31(+) staining were highest for cocultures at a 50:50 HMSC/HUVEC ratio. Therefore, the results from the present study show that a HMSC/HUVEC coculture ratio of 50:50 in OM is the best combination to obtain both osteogenic and angiogenic differentiation.