Components of cytochrome c oxidase detectable by EPR spectroscopy.
Components of cytochrome c oxidase detectable by EPR spectroscopy.
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可通过 EPR 光谱检测细胞色素 C 氧化酶的成分。
DOI：
10.1016/0005-2728(74)90178-9
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发表时间：
1974
期刊：
影响因子：
--
通讯作者：
Helmut Beinert
中科院分区：
文献类型：
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作者：
C. Hartzell;Helmut Beinert
描述了从冷冻牛心线粒体制备高血红素（⩽ 14μmoles/mg 蛋白质）、低外来铜（⩽ 1.1 原子铜/摩尔血红素）和低脂质（⩽ 0.05 g 磷脂/g 蛋白质）含量的细胞色素氧化酶（EC 1.9.3.1）的程序。详细描述了在 6 至 100 °K 之间的酶在各种氧化状态、不同 pH 条件和溶质存在条件下观察到的 EPR 信号。估计这些信号所代表的顺磁性物质的数量。在任何条件下，EPR 可检测物种的总和都不会超过大约。酶的 50% 的潜在顺磁性成分。与在多个物种的整个组织、线粒体和亚软骨颗粒中观察到的相应信号进行了比较。简要讨论了观察到的信号与细胞色素氧化酶已知成分的分配。
A procedure for the preparation from frozen beef heart mitochondria of cytochromecoxidase (EC 1.9.3.1) of high heme (⩽ 14μmoles/mg protein) and low extraneous copper (⩽ 1.1 atoms Cu/mole heme) and low lipid (⩽ 0.05 g phospholipid/g protein) content is described. EPR signals observed with the enzyme between 6 and 100 °K at various states of oxidation and at different conditions of pH and presence of solutes are described in detail. The quantities of paramagnetic species represented by these signals are estimated. Under no conditions does the sum of the EPR detectable species represent more than approx. 50% of the potentially paramagnetic components of the enzyme. Comparisons are made to the corresponding signals as observed in whole tissue, mitochondria and submitochondrial particles from a number of species. The assignment of the observed signals to known components of cytochromecoxidase is discussed briefly.