Periodontally diseased vs. normal roots as evaluated by scanning electron microscopy and electron probe analysis.
Periodontally diseased vs. normal roots as evaluated by scanning electron microscopy and electron probe analysis.
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通过扫描电子显微镜和电子探针分析评估患病牙根与正常牙根。
DOI：
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发表时间：
1987
期刊：
影响因子：
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通讯作者：
R. V. Van Swol
中科院分区：
文献类型：
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作者：
N. S. Barton;R. V. Van Swol
进行了扫描电子显微镜和电子探针研究，以比较牙周袋深处的根部结构与未表现出牙周疾病的根部。为了消除外来离子引入或缺失的可能性，在切片和在电子显微镜下观察之前，没有尝试对根进行固定、包埋或脱水。矿物质含量和浓度用电子探针在每颗牙齿的两个样本上测定。在一个样本上，仅分析了外牙骨质表面。在相邻的横截面上，从表面和根部的增量深度处读取读数。非参数统计分析比较了患病与非患病根。本研究得出以下结论：（1）用最少的样品制备，可以对人牙齿进行一致的、可重复的定性电子探针分析。这给出了更准确的评估，因为晶体结构的完整性没有被破坏。(2)矿物质一致发现P，Ca，Cu，Zn，Mg和Na。它们在整个分析区域的浓度相似。(3)Mg和Cu在未患病的牙齿中显示出较高的值。(4)暴露于牙周袋和非患病根之间的钙，磷，锌，钠的浓度没有差异。
A scanning electron microscope and electron probe study was carried out to compare root structure from deep within periodontal pockets with roots exhibiting no periodontal disease. To eliminate the possibility of extraneous ion introduction or deletion, no attempt was made to subject the roots to fixation, embedding, or dehydration prior to sectioning and viewing in the electron microscope. Mineral content and concentration were determined with an electron probe on two specimens per tooth. On one specimen, only the external cemental surface was analyzed. On an adjacent cross section, readings were taken from the surface and at incremental depths into the root. A nonparametric statistical analysis compared diseased with nondiseased roots. The following conclusions can be drawn from the study: (1) Consistent and repeatable qualitative electron probe analyses can be performed on human teeth with minimal specimen preparation. This gives a more accurate assessment, since the integrity of the crystalline structure is not disrupted. (2) Minerals consistently found were P, Ca, Cu, Zn, Mg and Na. They were in similar concentrations throughout the area analyzed. (3) Mg and Cu showed higher values in the nondiseased teeth. (4) There were no differences in concentrations for Ca, P, Zn, and Na between roots exposed to a periodontal pocket and nondiseased roots.