Systematic investigation and microbial community profile of indole degradation processes in two aerobic activated sludge systems.
Systematic investigation and microbial community profile of indole degradation processes in two aerobic activated sludge systems.
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两个好氧活性污泥系统吲哚降解过程的系统研究和微生物群落概况
DOI：
10.1038/srep17674
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发表时间：
2015-12-11
影响因子：
4.6
通讯作者：
Zhou J
中科院分区：
文献类型：
--
作者：
Ma Q;Qu Y;Zhang X;Liu Z;Li H;Zhang Z;Wang J;Shen W;Zhou J
吲哚广泛存在于各种环境基质中。吲哚降解菌的研究已有报道，而好氧微生物群落驱动的吲哚降解过程尚未见报道。以城市和焦化厂活性污泥为原料，构建了8个序批式生物反应器，用于吲哚的好氧处理。整个操作过程分为3个阶段，即第一阶段以葡萄糖和吲哚为碳源，第二阶段以吲哚为碳源，第三阶段以吲哚为碳源和氮源。吲哚在两种体系中均能被完全去除。Illumina测序结果显示，吲哚处理后α多样性降低，微生物群落在3个阶段间差异显著。在属的水平上，两个系统的第I阶段均以Azorcus和Thauera为优势种，市政污泥系统的第II阶段和第III阶段均以产碱菌属、丛毛单胞菌属和假单胞菌属为核心属，焦化污泥系统的第II阶段和第III阶段均以产碱菌属和伯克霍尔德菌属为核心属。此外，以吲哚为唯一碳源，分离到丛毛单胞菌属、伯克霍尔德菌属和嗜异性菌属的4株菌株。Burkholderia sp. IDO 3对100 mg/L吲哚的降解速度最快，可在14 h内完全去除。这些发现提供了新的信息，丰富了我们对吲哚好氧降解过程的理解。
Indole is widely spread in various environmental matrices. Indole degradation by bacteria has been reported previously, whereas its degradation processes driven by aerobic microbial community were as-yet unexplored. Herein, eight sequencing batch bioreactors fed with municipal and coking activated sludges were constructed for aerobic treatment of indole. The whole operation processes contained three stages, i.e. stage I, glucose and indole as carbon sources; stage II, indole as carbon source; and stage III, indole as carbon and nitrogen source. Indole could be completely removed in both systems. Illumina sequencing revealed that alpha diversity was reduced after indole treatment and microbial communities were significantly distinct among the three stages. At genus level, Azorcus and Thauera were dominant species in stage I in both systems, while Alcaligenes, Comamonas and Pseudomonas were the core genera in stage II and III in municipal sludge system, Alcaligenes and Burkholderia in coking sludge system. In addition, four strains belonged to genera Comamonas, Burkholderia and Xenophilus were isolated using indole as sole carbon source. Burkholderia sp. IDO3 could remove 100 mg/L indole completely within 14 h, the highest degradation rate to date. These findings provide novel information and enrich our understanding of indole aerobic degradation processes.