Flocculation in Saccharomyces cerevisiae is repressed by the COMPASS methylation complex during high-gravity fermentation

Flocculation in Saccharomyces cerevisiae is repressed by the COMPASS methylation complex during high-gravity fermentation
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DOI：
10.1002/yea.1643
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发表时间：
2008-12-01
期刊：
YEAST
影响因子：
2.6
通讯作者：
Brandt, Anders
Brandt, Anders
中科院分区：
生物学4区
文献类型：
--
作者：
Dietvorst, Judith;Brandt, Anders

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相似文献

研究了COMPASS在不同酿酒酵母菌株中沉默位于端粒附近的flo和mal基因的意义，这些酵母菌株以不同的效率发酵高浓度麦芽糖(20%)。在一株组成表达FLO11的快速麦芽糖发酵酵母中，FLO1、FLO5和FLO9在高浓度麦芽糖或葡萄糖的发酵过程中被诱导表达。另一株主要诱导表达FLO1。然而，指南针有缺陷的这些菌株的突变株在发酵早期形成了非常大的细胞聚集体，而且比野生型更明显。大的HoE的形成依赖于钙离子，并被甘露糖抑制。COMPASS基因缺陷突变株表现出的絮凝性是由于FLO1、FLO5和FLO9转录本数量增加所致。在发酵后期检测到COMPASS介导的MAL基因沉默，而在快速发酵高浓度麦芽糖的菌株中未检测到沉默。因此，除MAL基因外，COMPASS还参与沉默FLO1、FLO5和FLO9基因的表达。版权所有(C)2009 John Wiley&Sons，Ltd.
The significance of COMPASS on silencing of the FLO and MAL genes, located close to telomeres, was studied in different Saccharomyces cerevisiae strains that fermented high concentrations of maltose (20%) with different efficiency. In one particular fast maltose-fermenting yeast strain, with constitutive expression of FLO11, the expression of FLO1, FLO5 and FLO9 was induced during fermentation of high concentrations of maltose or glucose. In another strain the expression of mainly FLO1 was induced. Mutants of these strains with a defective COMPASS, however, formed very large aggregates of cells earlier in the fermentation and more pronounced than the wild-type. The formation of the large Hoes was dependent on calcium ions and was inhibited by mannose. The flocculation displayed by mutants defective in COMPASS was due to increased amounts of FLO1, FLO5 and FLO9 transcripts. COMPASS-mediated silencing of the MAL genes was detected at the later stages of fermentation in strains that fermented high concentrations of maltose slowly and incompletely, while silencing was not detectable in strains that fermented maltose fast. Thus, COMPASS, in addition to the MAL genes, is also involved in silencing the expression of the FLO1, FLO5 and FLO9 genes. Copyright (C) 2009 John Wiley & Sons, Ltd.