Estuarine molecular bycatch as a landscape-wide biomonitoring tool
Estuarine molecular bycatch as a landscape-wide biomonitoring tool
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河口分子兼捕物作为景观范围的生物监测工具
DOI：
10.1016/j.biocon.2021.109287
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发表时间：
2021
影响因子：
5.9
通讯作者：
Mariani S
中科院分区：
文献类型：
--
作者：
Mariani S
环境DNA分析正在迅速改变生物多样性监测，并支持全球范围内的保护应用。这种方法已被协助的元巴编码PCR引物，适合于检测范围广泛的类群的发展。然而，很少有人努力探索在每次调查中产生但随后被丢弃的非靶DNA序列的价值。在这里，我们表明，鱼类为目标的标记广泛应用于水生生物监测也可以检测鸟类和哺乳动物在周围的栖息地。我们展示了这一功能在三个温带河口在多个季节，数十种鸟类和哺乳动物物种提供了宝贵的见解，时空动物群的变化。我们的研究结果表明，现有的元条码序列数据集适合于挖掘和探索这种“分子附带捕获”，未来的基于eDNA的调查可以设计为适应这种广泛适用的工具的增强属性。
Environmental DNA analysis is rapidly transforming biodiversity monitoring and bolstering conservation applications worldwide. This approach has been assisted by the development of metabarcoding PCR primers that are suited for detection of a wide range of taxa. However, little effort has gone into exploring the value of the non-target DNA sequences that are generated in every survey, but subsequently discarded. Here we demonstrate that fish-targeted markers widely employed in aquatic biomonitoring can also detect birds and mammals present in the surrounding habitats. We showcase this feature in three temperate estuaries over multiple seasons, where dozens of bird and mammal species offer valuable insights into spatial and temporal faunal variation. Our results indicate that existing metabarcode sequence data sets are suitable for mining and exploration of this ‘molecular by-catch’, and that future eDNA-based surveys can be designed to accommodate this enhanced property of this widely applicable tool.
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