Gender difference in protein expression of vascular wall in mice exposed to chronic intermittent hypoxia: a preliminary study
Gender difference in protein expression of vascular wall in mice exposed to chronic intermittent hypoxia: a preliminary study
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慢性间歇性缺氧小鼠血管壁蛋白表达的性别差异初步研究
DOI：
10.4238/2014.october.20.25
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发表时间：
2014-01-01
影响因子：
0.4
通讯作者：
Wan, H. Y.
中科院分区：
文献类型：
--
作者：
Li, Q. Y.;Feng, Y.;Wan, H. Y.
阻塞性睡眠呼吸暂停（OSA）是心血管疾病的独立危险因素，如系统性动脉高血压、缺血性心脏病、中风、心力衰竭、心房颤动和心脏性猝死。阻塞性睡眠呼吸暂停患者心血管疾病的发病机制被认为主要是由慢性间歇性缺氧（CIH）引起的，CIH是睡眠期间氧合变化的一种特定模式。然而，CIH诱导的血管损伤和性别差异的潜在机制没有得到很好的记录。iTRAQ定量蛋白质组学方法能够分析几组中的许多不同蛋白质。因此，我们探讨了暴露于CIH的小鼠血管壁中蛋白质表达的性别差异。将每种性别的C57 BL/6 J小鼠暴露于CIH，吸入分数O-2（FiO（2））最低值为5%或对照，治疗时间为8 h/天，持续28天。使用iTRAQ蛋白质组学技术鉴定性别之间与CIH诱导的血管损伤相关的差异蛋白质。共鉴定出163种蛋白质，其中34种蛋白质在性别之间存在显著差异，这可能与CIH引起的血管损伤有关。与雄性小鼠相比，雌性小鼠中观察到20个上调蛋白和14个下调蛋白。我们发现不同性别的CIH下表达的血管蛋白，这表明这些蛋白可能是CIH血管损伤的生物标志物。
Obstructive sleep apnea (OSA) is an independent risk factor for cardiovascular diseases such as systemic arterial hypertension, ischemic heart disease, stroke, heart failure, atrial fibrillation, and cardiac sudden death. The pathogenesis of cardiovascular disease in OSA is thought to be induced primarily by chronic intermittent hypoxia (CIH), a specific pattern of change in oxygenation during sleep. However, the underlying mechanisms of CIH-induced vasculature injury and gender differences are not well documented. The iTRAQ Quantitative Proteomic method enables analysis of a number of different proteins among several groups. Thus, we explored gender differences in protein expression in the vascular walls of mice exposed to CIH. C57BL/6J mice of each gender were exposed to CIH with a fractional inspired O-2 (FiO(2)) nadir of 5% or control, with a treatment time of 8 h/day for 28 days. Differential proteins related to CIH-induced vascular injury between genders were identified using iTRAQ proteomic technology. A total of 163 proteins were identified, of which 34 showed significant differences between genders, which may correlate with vascular injury by CIH. Twenty up-regulated proteins and 14 downregulated proteins were observed in female mice compared with male mice. We identified different vascular proteins expressed under CIH between genders, suggesting that these proteins may be biomarkers of vascular injury by CIH.