Inhibition of fertility and sperm-zona binding by antiserum to the rabbit sperm membrane autoantigen RSA-1.
Inhibition of fertility and sperm-zona binding by antiserum to the rabbit sperm membrane autoantigen RSA-1.
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兔精子膜自身抗原 RSA-1 抗血清抑制生育力和精子带结合。
DOI：
10.1095/biolreprod25.3.621
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发表时间：
1981
影响因子：
3.6
通讯作者：
O'Rand,MG
中科院分区：
文献类型：
--
作者：
O'Rand,MG
采用兔精子膜自身抗原（RSA-1）特异性抗血清研究RSA-1在受精过程中的作用。用抗rsa -1 Fab处理过的精子进行人工授精，胎数减少68%。在体外，特异的Fab抗体降低了精子与带的结合（62%），并完全抑制了渗透。受精过程中RSA-1的免疫荧光定位表明，如果发生顶体反应，精子与透明带结合或穿透透明带时，精子的顶体后荧光消失。在卵泡周围游动的精子也没有荧光。由此可见，穿透和结合是独立的事件，可能涉及精子表面成分的不同但可能重叠的亚群，并且RSA-1在精子穿透透明带过程中起重要作用。
Specific antiserum to the rabbit sperm membrane autoantigen (RSA-1) was used to study the role of RSA-1 during fertilization. Artificial insemination of rabbits with spermatozoa treated with anti-RSA-1 Fab resulted in a 68% decrease in the number of fetuses. In vitro, sperm-zona binding was reduced (62%) and penetration completely inhibited by the specific Fab antibodies. Immunofluorescent localization of RSA-1 during fertilization showed that the postacrosomal fluorescence of the spermatozoon was lost on spermatozoa bound to the zona peliucida or penetrating it, if the acrosome reaction had occurred. Spermatozoa swimming in the perivitelline space also did not fluoresce. It is concluded that penetration and binding are independent events which may involve different but perhaps overlapping subsets of sperm surface components and that RSA-1 plays an important role during the penetration of the spermatozoon through the zona pellucida.