Cytosolic glucose-6-phosphate dehydrogenases play a pivotal role in Arabidopsis seed development.
Cytosolic glucose-6-phosphate dehydrogenases play a pivotal role in Arabidopsis seed development.
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DOI：
10.1016/j.plaphy.2022.07.017
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发表时间：
2022-07
期刊：
影响因子：
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通讯作者：
Mengjiao Ruan;Wenliang He;Hao Sun;Chaiyan Cui;Xiangxiang Wang;Ruiling Li;Xiaomin Wang;Y. Bi
中科院分区：
文献类型：
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作者：
Mengjiao Ruan;Wenliang He;Hao Sun;Chaiyan Cui;Xiangxiang Wang;Ruiling Li;Xiaomin Wang;Y. Bi
胚发育对种子产量和萌发后生长至关重要。葡萄糖-6-磷酸脱氢酶（Glucose-6-phosphate dehydrogenase, G6PD）是氧化戊糖磷酸途径（OPPP）中的限速酶，通过提供烟酰胺腺嘌呤二核苷酸磷酸（nictinamide adenine dinucleotide phosphate， NADPH）广泛参与植物发育和胁迫耐受。本研究利用胞质葡萄糖-6-磷酸脱氢酶（Cyt-G6PD）的双突变体（g6pd5/6）、过表达系（G6PD5/6OE）和互补系（g6pd5/6Comp），研究了Cyt-G6PD在拟南芥胚胎发育中的作用。结果表明，与Col-0相比，g6pd5/6种子的发芽率有所延迟；11.5%的g6pd5/6种子不发芽。Cyt-G6PD功能障碍导致g6pd5/6幼苗鲜重和主根长度下降。g6pd5/6植株的株高和株长也呈下降趋势。与Col-0、g6pd5/ 6oe和g6pd5/ 6complines相比，g6pd5/6植株的角质层和种子的败育率均有所提高。然而，Cyt-G6PD功能障碍不影响花粉活性；而在基因6pd5/6中，胚胎发育受到部分延迟或抑制。拟南芥种子中脂肪酸和贮藏蛋白这两种主要贮藏物质的含量在6pd5/6种子中显著降低。外源施用脂肪酸（C18:2; C18:3）减轻了g6pd5/6种子的延迟萌发。RT-qPCR结果进一步证实了早期胚胎发育基因6pd5/6的下调。综上所述，Cyt-G6PD通过调节早期胚胎发育基因的转录和储存物质（尤其是脂肪酸）的积累，在植物种子发育中起着关键作用。
Embryo development is essential for seed yield and post-germination growth. Glucose-6-phosphate dehydrogenase (G6PD), the rate-limiting enzyme in oxidative pentose phosphate pathway (OPPP), is widely involved in plant development and stress tolerance by providing nicotinamide adenine dinucleotide phosphate (NADPH). In this study, the double mutant (g6pd5/6), overexpression line (G6PD5/6OE) and complementation line (g6pd5/6Comp) of cytosolic glucose-6-phosphate dehydrogenases (Cyt-G6PD) were used to investigate Cyt-G6PD roles in embryo development of Arabidopsis. The results showed that the germination rate ofg6pd5/6seeds was delayed in comparison with that of Col-0; moreover, 11.5% ofg6pd5/6seeds did not germinate. The dysfunction of Cyt-G6PD resulted in decreased fresh weight and primary root length ofg6pd5/6seedlings. The height and silique length ofg6pd5/6plants were also decreased. Moreover, the abortion rate of siliques and seeds ofg6pd5/6plants were increased compared with those of Col-0, G6PD5/6OEandg6pd5/6Complines. However, the dysfunction of Cyt-G6PD did not affect pollen activity; but ing6pd5/6, the embryo development was partially delayed or inhibited. The contents of fatty acids and storage proteins, two main storage materials in Arabidopsis seeds, were decreased ing6pd5/6seeds. Exogenous application of fatty acids (C18:2; C18:3) alleviated the delayed germination ofg6pd5/6seeds. RT-qPCR results further demonstrated that the early embryo development genes were down-regulated ing6pd5/6. Taken together, Cyt-G6PD plays a pivotal role in plant seed development by regulating the transcriptions of early embryo development genes and the accumulation of storage materials (especially fatty acids).