Type III secretion homologs are present in Brucella melitensis, B-ovis, and B-suis biovars 1, 2, and 3
Type III secretion homologs are present in Brucella melitensis, B-ovis, and B-suis biovars 1, 2, and 3
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DOI：
10.1007/s00284-002-3789-3
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发表时间：
2003-04-01
影响因子：
2.6
通讯作者：
Winstanley, C
中科院分区：
文献类型：
--
作者：
Abdallah, AI;Commander, NJ;Winstanley, C
将来自表征的III型分泌（TTS）系统的蛋白质序列用作计算机模拟探针，以鉴定猪布鲁氏菌生物型1菌株1330基因组序列中的几个TTS基因同源物。四个基因，命名为flhB，fliP，fliR，和fliF的基础上最大的同源性已知鞭毛器蛋白，有针对性的PCR和杂交试验，以确定其分布在其他布鲁氏菌命名物种和biovars。结果表明，flhB、fliP、fliR和fliF存在于羊种布鲁氏菌、绵羊种布鲁氏菌和猪种布鲁氏菌生物变型1、2和3中。以前在流产布鲁氏菌中也报道过类似的同源物。使用RT-PCR检测，我们无法检测到这些基因的任何表达。目前尚不清楚这些基因是否是鞭毛系统的隐藏残留物，或者是否积极参与致病性或以前未检测到的运动性过程，但它们在布鲁氏菌中广泛分布，值得进一步研究以确定它们的作用。
Protein sequences from characterized type III secretion (TTS) systems were used as probes in silico to identify several TTS gene homologs in the genome sequence of Brucella suis biovar 1 strain 1330. Four of the genes, named flhB, fliP, fliR, and fliF on the basis of greatest homologies to known flagellar apparatus proteins, were targeted in PCR and hybridization assays to determine their distribution among other Brucella nomen species and biovars. The results indicated that flhB, fliP, fliR and fliF are present in Brucella melitensis, Brucella ovis, and Brucella suis biovars 1, 2 and 3. Similar homologos have been reported previously in Brucella abortus. Using RT-PCR assays, we were unable to detect any expression of these genes. It is not yet known whether the genes are the cryptic remnants of a flagellar system or are actively involved in a process contributing to pathogenicity or previously undetected motility, but they are distributed widely in Brucella and merit further study to determine their role.