The adsorption protein of bacteriophage fd and its neighbour minor coat protein build a structural entity.
The adsorption protein of bacteriophage fd and its neighbour minor coat protein build a structural entity.
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噬菌体fd的吸附蛋白与其邻近的次要外壳蛋白构建了一个结构实体。
DOI：
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Ihab Rasched
中科院分区：
文献类型：
--
作者：
V. Gailus;Ihab Rasched
吸附蛋白G3P和另一个次要的外壳蛋白G6P位于丝状噬菌体FD[Grant，R.A.，Lin，T.C.，Konigsberg，W.E.&Webster，R.E.(1981)J.Biol]的一端。化学。256,539-546]。这两种蛋白质代表近端，通过一种允许温和增溶的技术作为一个实体分离。用洗涤剂脱氧胆酸钠和氯仿破坏噬菌体颗粒，解离主要的外壳蛋白g8p，释放噬菌体DNA，但留下g3p和g6p相互关联。在脱氧胆酸盐存在下，用凝胶过滤层析分离纯化了g3p-G6P络合物和低相对分子质量的g3p低聚体。这些不同的g3p寡聚结构在非变性聚丙烯酰胺凝胶电泳法中表现出不同的迁移率。从脱氧胆酸盐和Triton-X-100溶解的非变性聚丙烯酰胺凝胶电泳法中也发现了这两种形式，而不需要事先进行层析分离。对于低分子质量物种，g3p的两种低聚形式由两条g3p多肽链组成；对于吸附络合物，g3p和G6p的两条寡聚体由四条g3p和四条g6p多肽链组成。
The adsorption protein g3p and another minor coat protein, g6p, are located at one end of the filamentous bacteriophage fd [Grant, R.A., Lin, T.C., Konigsberg, W.E. & Webster, R.E. (1981) J. Biol. Chem. 256, 539-546]. Both proteins, representing the proximal tip, were detached as an entity by a technique that allowed for gentle solubilization. Disrupting the phage particle with the detergent sodium deoxycholate and chloroform dissociates the major coat protein g8p, frees the phage DNA, but leaves g3p and g6p associated with each other. The g3p-g6p complex, which we termed the adsorption complex, and an oligomeric form of g3p with lower molecular mass were isolated and purified by gel-filtration chromatography in the presence of deoxycholate. These different oligomeric structures of g3p showed a different mobility in non-denaturing polyacrylamide-gel electrophoresis. Both forms were also found in non-denaturing polyacrylamide-gel electrophoresis from deoxycholate- and Triton-X-100-solubilized phage without prior chromatographic separation. The two oligomeric forms of g3p are composed of two g3p polypeptide chains in the case of the low-molecular-mass species, and four g3p and four g6p polypeptide chains for the adsorption complex.
丝状噬菌体 fl 和 fd 中基因 IX 和基因 VI 编码的 C 和 D 蛋白的分离和表征。
DOI：
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发表时间：
1980
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Lin,TC;Webster,RE;Konigsberg,W
通讯作者：
Konigsberg,W
丝状噬菌体 fl 的结构。
DOI：
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发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Grant,RA;Lin,TC;Konigsberg,W;Webster,RE
通讯作者：
Webster,RE
影响因子：
2.9
作者：
BLAKE, MS;JOHNSTON, KH;GOTSCHLICH, EC
通讯作者：
GOTSCHLICH, EC