The Madness of Microbiome: Attempting To Find Consensus "Best Practice" for 16S Microbiome Studies.
The Madness of Microbiome: Attempting To Find Consensus "Best Practice" for 16S Microbiome Studies.
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DOI：
10.1128/aem.02627-17
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发表时间：
2018-04-01
影响因子：
4.4
通讯作者：
Watson M
中科院分区：
文献类型：
--
作者：
Pollock J;Glendinning L;Wisedchanwet T;Watson M
高通量测序平台的发展和不断改进激发了人们对复杂微生物群落研究的兴趣。目前，研究微生物群落组成和动态的最流行的测序方法是靶向16S rRNA基因元巴编码。为了制备用于测序的样品，存在多种处理步骤，每种步骤都有可能在数据分析阶段引入偏倚。在这篇简短的综述中，描述了与每个加工步骤相关的文献中的关键信息，因此，对未来16 S rRNA基因元巴编码实验提出了一般建议。
The development and continuous improvement of high-throughput sequencing platforms have stimulated interest in the study of complex microbial communities. Currently, the most popular sequencing approach to study microbial community composition and dynamics is targeted 16S rRNA gene metabarcoding. To prepare samples for sequencing, there are a variety of processing steps, each with the potential to introduce bias at the data analysis stage. In this short review, key information from the literature pertaining to each processing step is described, and consequently, general recommendations for future 16S rRNA gene metabarcoding experiments are made.