Oral eicosapentaenoic acid supplementation as possible therapy for endometriosis
Oral eicosapentaenoic acid supplementation as possible therapy for endometriosis
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DOI：
10.1016/j.fertnstert.2007.08.014
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发表时间：
2008-10-01
影响因子：
6.7
通讯作者：
Suzuki, Mitsuaki
中科院分区：
文献类型：
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作者：
Netsu, Sachiho;Konno, Ryo;Suzuki, Mitsuaki
目的：比较n-3二十碳五烯酸（EPA）与n-6亚油酸（LA）在子宫内膜异位症大鼠模型中的抗炎作用。基于脂质摄入不平衡是近期子宫内膜异位症增加的因素之一的假设，我们重点研究了子宫内膜异位症中脂质代谢与炎症反应之间的关系。设计：前瞻性、随机实验研究。地点：大学附属医院动物外科实验室。动物：Sprague-Dawley大鼠（雌性，6周龄）。干预(S)：给大鼠喂食EPA （n = 9）或LA (n = 9)，为期2周。喂养2周后，将子宫自体移植至腹膜，建立子宫内膜异位症模型。连续饲喂6周。自体移植后2周和4周，每组处死3只大鼠进行评价。主要观察指标：对子宫内膜异位症病变进行形态学评估，并检测其脂肪酸组成。采用cDNA微阵列分析和实时定量逆转录聚合酶链反应（RT-PCR）对这些组织中的基因表达进行评估。结果：EPA组各组织n-3:n-6比值显著升高，子宫内膜异位症炎症活性部位间质增厚明显受到抑制（EPA组0.30 +/- 0.09 mm vs LA组0.77 +/- 0.23 mm）。EPA组金属蛋白酶、白细胞介素-1 β、白细胞介素-1r、前列腺素E合成酶（Ptges）、核因子(NF)- κ B mRNA表达均降低。结论：补充EPA可能是治疗子宫内膜异位症的有效策略。
Objective: To investigate the anti-inflammatory effect of n-3 eicosapentaenoic acid (EPA) compared with n-6 linoleic acid (LA) in an endometriosis rat model. We focused on the relationship between lipid metabolism and inflammatory reactions in endometriosis based on the hypothesis that a lipid intake imbalance is one of the factors responsible for the recent increase of endometriosis. Design: Prospective, randomized experimental study. Setting: Animal surgery laboratory in a university hospital. Animal(s): Sprague-Dawley rats (female, 6 weeks old). Intervention(S): Rats were fed a diet with EPA (n = 9) or with LA (n = 9) for 2 weeks. Two weeks after feeding, the uterus was autotransplanted to the peritoneum to construct an endometriosis model. Feeding was continued for a total of 6 weeks. Two and 4 weeks after autotransplantation, three rats of each group were killed and evaluated. Main Outcome Measure(s): Endometriotic lesions were morphologically evaluated and their fatty acid composition was examined. Gene expression in these tissues was evaluated by cDNA microarray analysis and quantative real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Result(s): in the EPA group, the n-3:n-6 ratio in each tissue significantly increased and the thickening of the interstitium, an active site for inflammation in endometriosis, was significantly suppressed (0.30 +/- 0.09 mm [EPA group] vs. 0.77 +/- 0.23 mm [LA group]). The mRNA of metalloproteinases, interleukin-1 beta, interleukin-1r, prostaglandin E synthase (Ptges), and nuclear factor (NF)-kappa B were reduced in the EPA group. Conclusion(s): EPA supplementation might be a valid strategy for the treatment of endometriosis.