A highly divergent picornavirus in a marine mammal
A highly divergent picornavirus in a marine mammal
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DOI：
10.1128/jvi.01240-07
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发表时间：
2008-01-01
影响因子：
5.4
通讯作者：
Delwart, E.
中科院分区：
文献类型：
--
作者：
Kapoor, A.;Victoria, J.;Delwart, E.
从环斑海豹（Phoca hispida）的一种未知病毒的核酸扩增使用序列独立的PCR，亚克隆，然后测序。一种新的RNA病毒的全基因组被推导出来，在海洋哺乳动物中鉴定出第一个序列确认的小核糖核酸病毒。暂时命名为海豹小核糖核酸病毒I（SePV-1）作为一个离群的双埃柯病毒的分组的系统发育位置被发现一致的基因组的可识别区域。SePV-1的3D聚合酶基因序列与其他小核糖核酸病毒的3D聚合酶基因序列之间的平均蛋白质序列同一性仅为19.3%至30.0%。短的506个碱基的5 '-非翻译区的预测二级结构显示了IVB型内部核糖体进入位点的一些属性，并且多聚蛋白缺乏明显的L肽，这两种性质都与副肠孤病毒属相关。两个SePV-1 2A基因和共翻译切割所需的典型序列的存在类似于Ljungan病毒的遗传组织。在2000年至2002年收集的108只海豹中有8只（7.4%）的培养上清液中检测到微小的遗传变异，表明SePV-1在这一狩猎海豹种群中的流行率很高。与其他小核糖核酸病毒相比，SePV-1的高水平遗传趋异及其与其最近亲属的特征混合支持SePV-1作为小核糖核酸病毒科中新属的原型的临时分类。
Nucleic acids from an unidentified virus from ringed seals (Phoca hispida) were amplified using sequence-independent PCR, subcloned, and then sequenced. The full genome of a novel RNA virus was derived, identifying the first sequence-confirmed picornavirus in a marine mammal. The phylogenetic position of the tentatively named seal picornavirus I (SePV-1) as an outlier to the grouping of parechoviruses was found consistently in alignable regions of the genome. A mean protein sequence identity of only 19.3 to 30.0% was found between the 3D polymerase gene sequence of SePV-1 and those of other picornaviruses. The predicted secondary structure of the short 506-base 5'-untranslated region showed some attributes of a type IVB internal ribosome entry site, and the polyprotein lacked an apparent L pepticle, both properties associated with the Parechovirus genus. The presence of two SePV-1 2A genes and of the canonical sequence required for cotranslational cleavage resembled the genetic organization of Ljungan virus. Minor genetic variants were detected in culture supernatants derived from 8 of 108 (7.4%) seals collected in 2000 to 2002, indicating a high prevalence of SePV-1 in this hunted seal population. The high level of genetic divergence of SePV-1 compared to other picornaviruses and its mix of characteristics relative to its closest relatives support the provisional classification of SePV-1 as the prototype for a new genus in the family Picornaviridae.