A novel genetic variant of the goat Six6 gene and its association with production traits in Chinese goat breeds.
A novel genetic variant of the goat Six6 gene and its association with production traits in Chinese goat breeds.
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DOI：
10.4238/2011.november.22.9
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发表时间：
2011-11
期刊：
影响因子：
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通讯作者：
C. Pan;X. Lan;H. Zhao;S. R. Hu;Y. Huai;Chuzhao Lei;Hong Chen
中科院分区：
文献类型：
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作者：
C. Pan;X. Lan;H. Zhao;S. R. Hu;Y. Huai;Chuzhao Lei;Hong Chen
我们通过聚合酶链反应 - 单链构象多态性（PCR - SSCP）、DNA测序和强制限制性片段长度多态性 - 聚合酶链反应（forced RFLP - PCR）在Six6基因内寻找新的遗传变异，并评估它们与中国8个本土品种的2132只山羊生产性状的关联。在山羊Six6基因内鉴定出一种新的单核苷酸多态性（NM_001104993.1: g.232T>C）。等位基因“C”的频率在0.8621到1.000之间变化，且处于哈迪 - 温伯格平衡。发现各品种的基因型和等位基因频率存在显著差异。关联分析显示，在海南黑山羊中基因型与管围之间存在显著关系（P = 0.047）；基因型为CT的成年个体比基因型为CC的个体具有更高的管围。在内蒙古白绒山羊中发现基因型与5岁羊绒长度存在另一种显著关联（P = 0.002）。此外，基因型为CT的个体比基因型为CC的个体具有更长的羊绒长度。数据表明Six6基因对生长性状和羊绒性状有积极影响。Six6 - PstI的多态性可作为通过标记辅助选择进行山羊育种和遗传学研究的DNA标记。
We looked for novel genetic variations within the Six6 gene by PCR-SSCP, DNA sequencing and forced RFLP-PCR and estimated their associations with production traits in 2132 goats of eight indigenous Chinese breeds. A novel single nucleotide polymorphism (NM_001104993.1: g.232T>C) within the goat Six6 gene was identified. The frequencies of allele "C" varied from 0.8621 to 1.000, which were in Hardy-Weinberg equilibrium. Genotype and allele frequencies were found to be significantly different in the breeds. Association analysis revealed a significant relationship between genotypes and cannon circumference in Hainan black goats (P = 0.047); adult individuals with genotype CT showed higher cannon circumference than those with genotype CC. Another significant association of genotypes with five-year-old fiber length was found in Inner Mongolia white cashmere goats (P = 0.002). In addition, individuals with genotype CT had longer fiber length than those with genotype CC. The data revealed that the Six6 gene positively affects growth traits and cashmere traits. Polymorphism of Six6-PstI could be useful as a DNA marker for goat breeding and genetics via marker-assisted selection.