Electrochemical detection of DNA hybridization based on DNA-templated assembly of silver cluster
Electrochemical detection of DNA hybridization based on DNA-templated assembly of silver cluster
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DOI：
10.1016/s1388-2481(02)00542-8
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发表时间：
2003
影响因子：
5.4
通讯作者：
Joseph Wang;O. Rincón;Ronen Polsky;E. Domínguez
中科院分区：
文献类型：
--
作者：
Joseph Wang;O. Rincón;Ronen Polsky;E. Domínguez
金属在DNA模板上的生长引起了人们对金属纳米结构设计的极大兴趣。在这里，我们利用DNA诱导的金属团簇的产生来开发一种电子生物传感协议。新的杂交分析采用了探针修饰的金表面，基于沿着DNA双链的银离子的静电收集，沿着DNA主干的银纳米簇的还原形成，银聚集体的溶解，以及在厚膜碳电极上溶解的银的溶出电位检测。因此，新的方案将剥离分析的固有信号放大与对非杂交DNA的有效区分结合在一起。
The growth of metals on DNA templates has generated considerable interest in connection to the design of metallic nanostructures. Here we exploit the DNA-induced generation of metal clusters for developing an electrical biosensing protocol. The new hybridization assay employs a probe-modified gold surface, and is based on the electrostatic ‘collection’ of silver cations along the DNA duplex, the reductive formation of silver nanoclusters along the DNA backbone, dissolution of the silver aggregate and stripping potentiometric detection of the dissolved silver at a thick-film carbon electrode. The new protocol thus combines the inherent signal amplification of stripping analysis with effective discrimination against nonhybridized DNA.