Effects of sodium saccharin and caffeine on the urinary bladder of rats treated with n-butyl-n-(4-hydroxybutyl)nitrosamine.
Effects of sodium saccharin and caffeine on the urinary bladder of rats treated with n-butyl-n-(4-hydroxybutyl)nitrosamine.
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糖精钠和咖啡因对正丁基-正(4-羟丁基)亚硝胺处理的大鼠膀胱的影响。
DOI：
10.20772/cancersci1959.71.4_490
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
N. Ito
中科院分区：
文献类型：
--
作者：
K. Nakanishi;M. Hirose;T. Ogiso;R. Hasegawa;M. Arai;N. Ito
研究了糖精钠和咖啡因对n -丁基- n -（4-羟基丁基）亚硝胺（BBN）诱导Wistar大鼠膀胱癌发生的影响。采用0.01% BBN作为启动剂4周，然后糖精钠和/或咖啡因作为启动剂32周（实验一），或0.001% BBN、糖精钠和/或咖啡因同时处理40周（实验二）。然后取出膀胱，用光镜和电子显微镜检查。与单独给药相比，BBN后序贯给药糖精钠显著增强了对增生的诱导作用（实验一），与BBN同时给药糖精钠比单独给药BBN显著增强了对增生和乳头状瘤的诱导作用（实验二）。在两个实验中，糖精钠单独处理的大鼠膀胱出现了两种类型的增生。单独使用咖啡因对大鼠膀胱上皮没有影响，在这些实验中，连续或同时使用咖啡因与BBN均未引起明显的癌变增强。
The effects of sodium saccharin and caffeine on urinary bladder carcinogenesis in Wistar strain rats treated with N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN) were studied. Animals were given 0.01% BBN as an initiator for 4 weeks and then sodium saccharin and/or caffeine as promoters for 32 weeks (Experiment I), or were treated simultaneously with 0.001% BBN, sodium saccharin and/or caffeine for 40 weeks (Experiment II). The urinary bladders were then removed and examined by light and electron microscopy. Sequential administration of sodium saccharin after BBN significantly enhanced the induction of hyperplasias compared with administration of BBN alone (Experiment I), and simultaneous administration of sodium saccharin with BBN significantly enhanced the induction of hyperplasia and papillomas compared with BBN alone (Experiment II). Two types of hyperplasias developed in the urinary bladder of rats treated with sodium saccharin alone in both experiments. Caffeine alone had no effect on the rat urinary bladder epithelium, and either sequential or simultaneous administration of caffeine with BBN caused no marked enhancement of carcinogenesis in these experiments.