Platelet Lysate-Modified Porous Silicon Microparticles for Enhanced Cell Proliferation in Wound Healing Applications
Platelet Lysate-Modified Porous Silicon Microparticles for Enhanced Cell Proliferation in Wound Healing Applications
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DOI：
10.1021/acsami.5b10950
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发表时间：
2016-01-13
影响因子：
9.5
通讯作者：
Santos, Helder A.
中科院分区：
文献类型：
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作者：
Fontana, Flavia;Mori, Michela;Santos, Helder A.
治疗慢性不愈合伤口的新前沿是使用微颗粒和纳米颗粒将药物或生长因子输送到伤口中。在这里，我们使用血小板裂解液（PL），一种血小板的血液衍生物，由生长因子的多因子混合物组成，来修饰多孔硅（PSi）微粒，并在体外和离体评估所开发的微系统的特性。评估了pl修饰的PSi诱导成纤维细胞增殖的潜力。然后在体外伤口愈合试验中评估微系统促进伤口愈合的特性。最后，在人体皮肤的离体实验中对pl修饰的PSi微粒进行了评估。结果表明，在不同的实验条件下，pl修饰的PSi微颗粒具有细胞相容性，并能促进细胞增殖。此外，在伤口愈合实验中，该微系统在与阳性对照相当的时间内促进了成纤维细胞之间间隙的关闭，并在离体实验中诱导了人体皮肤的增殖和再生过程。总之，我们的研究结果表明，pl修饰的PSi微粒子是一种适合进一步发展的微系统，可用于治疗慢性不愈合伤口。
The new frontier in the treatment of chronic nonhealing wounds is the use of micro- and nanoparticles to deliver drugs or growth factors into the wound. Here, we used platelet lysate (PL), a hemoderivative of platelets, consisting of a multifactorial cocktail of growth factors, to modify porous silicon (PSi) microparticles and assessed both in vitro and ex vivo the properties of the developed microsystem. PL-modified PSi was assessed for its potential to induce proliferation of fibroblasts. The wound closure-promoting properties of the microsystem were then assessed in an in vitro wound healing assay. Finally, the PL-modified PSi microparticles were evaluated in an ex vivo experiment over human skin. It was shown that PL-modified PSi microparticles were cytocompatible and enhanced the cell proliferation in different experimental settings. In addition, this microsystem promoted the closure of the gap between the fibroblast cells in the wound healing assay, in periods of time comparable with the positive control, and induced a proliferation and regeneration process onto the human skin in an ex vivo experiment. Overall, our results show that PL-modified PSi microparticles are suitable microsystems for further development toward applications in the treatment of chronic nonhealing wounds.