A simplified method of detection of clonal rearrangements of the T-cell receptor-gamma chain gene.
A simplified method of detection of clonal rearrangements of the T-cell receptor-gamma chain gene.
复制标题
DOI：
10.1097/00019606-199209000-00003
复制
发表时间：
1992-09-01
期刊：
影响因子：
--
通讯作者：
Wiedemann, L M
中科院分区：
文献类型：
--
作者：
McCarthy, K P;Sloane, J P;Wiedemann, L M
对外周血、骨髓或组织样本中的一系列 T 细胞增殖以及七种 T 细胞系进行了克隆性分析。所使用的技术采用聚合酶链式反应 (PCR) 来扩增重排的 T 细胞受体 γ 基因，使用引物识别可变序列中的保守序列并连接基因片段。在分析的 20 例 T 细胞白血病或淋巴瘤病例中，在 14 例 (70%) 中检测到克隆：在 7 个 T 细胞系中，在 6 例 (84%) 中检测到克隆。八种非 T 细胞疾病（包括非淋巴恶性肿瘤和反应性疾病）未记录到阳性结果。当这项技术的结果与我们之前发表的使用 PCR 检测克隆重排 T 细胞受体-β (TCR-β) 基因的方法的结果相结合时，检测到 10 个 T 细胞肿瘤中的 9 个 (90%)。该方法仅在两管中使用四种引物组合，因此简单快速：不需要放射性标记，仅使用少量组织，并且可以在福尔马林固定、石蜡包埋的组织上进行。
A series of T-cell proliferations in peripheral blood, bone marrow, or tissue samples, together with seven T-cell lines, were analysed for clonality. The technique used employs the polymerase chain reaction (PCR) to amplify rearranged T-cell receptor gamma genes, using primers recognising conserved sequences in the variable and joining gene segments. Of the 20 cases of T-cell leukaemia or lymphoma analysed, a clone was detected in 14 (70%): Of seven T-cell lines, a clone was detected in 6 (84%). No positive results were recorded in eight non-T-cell disorders (including nonlymphoid malignancies and reactive disorders). When the results of this technique were combined with the results of our previously published method for the detection of clonally rearranged T-cell receptor-beta (TCR-beta) genes using PCR, 9 of 10 (90%) T-cell tumours were detected. This method uses only four primer combinations in two tubes, and is therefore simple and rapid: it requires no radiolabelling, uses only a small amount of tissue, and can be performed on formalin-fixed, paraffin-embedded tissue.