Characterization of DNA structures by circular dichroism.
Characterization of DNA structures by circular dichroism.
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DOI：
10.1002/0471142700.nc0711s11
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发表时间：
2003-02-01
影响因子：
--
通讯作者：
Chaires, Jonathan B
中科院分区：
文献类型：
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作者：
Bishop, G Reid;Chaires, Jonathan B
核酸的圆二色性 (CD) 通常用于提供给定二级结构的特征。 CD 对于监测因温度、离子强度和 pH 等环境条件变化而引起的结构变化特别有效。许多市售仪器可用于测量圆二色光谱。由于每台机器的仪器操作细节有所不同，因此该单元提供描述性指南，而不是严格的协议。它描述了实验者在尝试通过圆二色分光偏振法检查核酸结构时将面临的考虑因素。还给出了细胞填充和清洁以及仪器校准的具体细节。最后，给出了 CD 谱的几个例子，为各种核酸结构（从单链到三链体和四链体形式）提供有用的参考点。
Circular dichroism (CD) of nucleic acids is commonly used to provide a signature for a given secondary structure. CD is particularly powerful for monitoring structural changes resulting from changes in environmental conditions such as temperature, ionic strength, and pH. A number of commercially available instruments are available for the measurement of CD spectra. Since the details of instrumental operation differ for each machine, this unit provides a descriptive guide, rather than a strict protocol. It describes considerations that the experimentalist will face when attempting to examine nucleic acid structures by CD spectropolarimetry. Specific details are also given for cell filling and cleaning, and for instrumental calibration. Finally, several examples of CD spectra are given to provide useful reference points for a variety of nucleic acid structures, ranging from single-stranded to triplex and quadruplex forms.