Involvement of a specific protein in the regulation of a circadian rhythm in Aplysia eye.
Involvement of a specific protein in the regulation of a circadian rhythm in Aplysia eye.
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特定蛋白质参与海兔眼昼夜节律的调节。
DOI：
10.1073/pnas.84.1.279
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发表时间：
1987
影响因子：
11.1
通讯作者：
Eskin,A
中科院分区：
文献类型：
--
作者：
Yeung,SJ;Eskin,A
我们以前的研究结果表明，蛋白质合成是必需的5-羟色胺（5-HT），以调节生物钟的相位在失智症的眼睛。此外，我们发现，5-HT似乎增加了一个34 kDa的蛋白质的合成，等电点为7.2。随后的研究进行了定量的影响，5-HT的34-kDa蛋白，并检查是否34-kDa蛋白参与昼夜节律计时系统。研究了5-HT对34-kDa蛋白影响的区域特异性。眼睛的近端部分似乎比远端部分合成更多的34-kDa蛋白质。此外，5-HT有一个更大的影响，在近端部分比在远端部分的34 kDa蛋白质的合成。合成的近端位置和5-HT对34-kDa蛋白质合成的影响与昼夜节律表达所需的细胞和过程的近端位置相关。还研究了5-HT对昼夜节律的影响与5-HT对34-kDa蛋白的影响之间的关系。由于5-HT通过激活腺苷酸环化酶以增加cAMP而引起节律的相移，因此毛喉素和8-苄硫腺苷3 '，5'-环一磷酸模拟了5-HT对34-kDa蛋白的作用。我们还发现，5-HT显着增加合成的34-kDa的蛋白质的相位时，5-HT延迟或提前的相位的节奏，但没有增加合成的34-kDa的蛋白质的相位时，5-HT没有相移。5-HT对34-kDa蛋白质的这种相位依赖性作用定性地解释了5-HT对昼夜节律的作用的相位依赖性。这些结果，当考虑与我们以前的数据，表明34 kDa蛋白质的合成直接参与5-HT产生的相移。34 kDa蛋白是值得未来的调查作为一个候选人的组成部分的昼夜节律振荡器。
Our previous results indicated that protein synthesis was necessary for serotonin (5-hydroxytryptamine, 5-HT) to regulate the phase of the biological clock in the Aplysia eye. Also, we showed that 5-HT appeared to increase the synthesis of a 34-kDa protein with an isoelectric point of 7.2. Subsequent studies were carried out to quantitate the effect of 5-HT on the 34-kDa protein and to examine whether the 34-kDa protein was involved in the circadian timing system. The regional specificity of the effect of 5-HT on the 34-kDa protein was investigated. The proximal portion of the eye appeared to synthesize much more of the 34-kDa protein than the distal portion. Also, 5-HT had a much larger effect on the synthesis of the 34-kDa protein in the proximal portion than in the distal portion. The proximal location of synthesis and the 5-HT effect on the synthesis of the 34-kDa protein correlate with the proximal location of cells and processes that are necessary for the expression of the circadian rhythm. The relationship between the effect of 5-HT on the circadian rhythm and the effect of 5-HT on the 34-kDa protein was also examined. As 5-HT causes phase shifts in the rhythm by activating adenylate cyclase to increase cAMP, forskolin and 8-benzylthioadenosine 3',5'-cyclic monophosphate mimicked the effect of 5-HT on the 34-kDa protein. We also found that 5-HT significantly increased the synthesis of the 34-kDa protein at phases when 5-HT delays or advances the phase of the rhythm but did not increase the synthesis of the 34-kDa protein at a phase when 5-HT did not phase shift. This phase-dependent effect of 5-HT on the 34-kDa protein qualitatively accounts for the phase dependence of the effect of 5-HT on the circadian rhythm. These results, when considered together with our earlier data, suggest that the synthesis of the 34-kDa protein is directly involved in the phase shift produced by 5-HT. The 34-kDa protein is worthy of future investigation as a candidate for a component of the circadian oscillator.