Mammalian transgenesis by intracytoplasmic sperm injection.
Mammalian transgenesis by intracytoplasmic sperm injection.
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DOI：
10.1097/00006254-199910000-00018
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发表时间：
1999-10
期刊：
影响因子：
56.9
通讯作者：
Anthony C.F. Perry;Teruhiko Wakayama;H. Kishikawa;Tsuyoshi Kasai;Masaru Okabe;Yutaka Toyoda;Ryuzo Yanagimachi
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文献类型：
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作者：
Anthony C.F. Perry;Teruhiko Wakayama;H. Kishikawa;Tsuyoshi Kasai;Masaru Okabe;Yutaka Toyoda;Ryuzo Yanagimachi
将未受精的小鼠卵母细胞与精子头和编码绿色荧光蛋白 (GFP) 或 β-半乳糖苷酶报告基因的外源 DNA 共注射，产生了 64% 至 94% 的转基因表达胚胎，反映了共注射前 DNA 与精子头的关联。将 GFP 系列胚胎非选择性转移至代孕母亲，产生了约 20% 的表达整合转基因的后代。这些数据表明，外源 DNA 可以通过显微注射的精子可重复地递送至卵母细胞中，并提出了一种适应性强的转基因方法。
Coinjection of unfertilized mouse oocytes with sperm heads and exogenous DNA encoding either a green fluorescent protein (GFP) or beta-galactosidase reporter produced 64 to 94 percent transgene-expressing embryos, reflecting DNA-sperm head association before coinjection. Nonselective transfer to surrogate mothers of embryos in the GFP series generated about 20 percent offspring expressing the integrated transgene. These data indicate that exogenous DNA can reproducibly be delivered into an oocyte by microinjected spermatozoa and suggest an adaptable method of transgenesis.