MURINE MODEL FOR EVALUATION OF PROTECTIVE IMMUNITY TO INFLUENZA-VIRUS
MURINE MODEL FOR EVALUATION OF PROTECTIVE IMMUNITY TO INFLUENZA-VIRUS
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DOI：
10.1016/0264-410x(93)90339-y
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发表时间：
1993-01-01
期刊：
影响因子：
5.5
通讯作者：
COMPANS, RW
中科院分区：
文献类型：
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作者：
NOVAK, M;MOLDOVEANU, Z;COMPANS, RW
我们将小鼠模型描述为一种廉价、易得和敏感的动物模型，用于评价各种甲型流感疫苗制剂给药途径诱导的保护性免疫应答。使用非小鼠适应的人流感病毒鼻内感染未麻醉的动物，我们确定了Balb/c小鼠感染的最佳剂量为10（4）个病毒空斑形成单位，测定呼吸道器官中病毒产量的最佳采样时间为攻毒后72 h。我们发现感染是从鼻子开始的，并在几天内下降到气管和肺部。抗感染保护的评价清楚地表明，小鼠呼吸道组织在鼻内给予全灭活病毒后得到完全保护，而在皮下给予病毒时得到部分保护。这种保护作用与唾液中的病毒特异性伊加抗体水平有关。
We have characterized a murine model as an inexpensive, readily available and sensitive animal model for the evaluation of protective immune responses induced by various routes of administration of influenza A vaccine preparations. Using a non-mouse-adapted human influenza virus to infect unanaesthetized animals intranasally, we established that the optimum dose for infection of Balb/c mice was 10(4) plaque forming units of virus and that the optimum sampling time for measurement of virus yields in the organs of the respiratory tract was 72 h after challenge. We found that the infection was initiated in the nose and progressed by descending into the trachea and lungs over a period of days. Evaluation of protection against infection clearly showed that the tissues of the mouse respiratory tract were completely protected after administration of whole killed virus intranasally and partly protected when virus was administered subcutaneously. The protection correlated with the level of virus-specific IgA antibodies in saliva.