EXPRESSION OF CDK5 (PSSALRE KINASE), A NEURAL CDC2-RELATED PROTEIN-KINASE, IN THE MATURE AND DEVELOPING MOUSE CENTRAL AND PERIPHERAL NERVOUS SYSTEMS
EXPRESSION OF CDK5 (PSSALRE KINASE), A NEURAL CDC2-RELATED PROTEIN-KINASE, IN THE MATURE AND DEVELOPING MOUSE CENTRAL AND PERIPHERAL NERVOUS SYSTEMS
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DOI：
10.1016/0006-8993(94)91197-5
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发表时间：
1994-10-24
期刊：
影响因子：
2.9
通讯作者：
TATIBANA, M
中科院分区：
文献类型：
--
作者：
INO, H;ISHIZUKA, T;TATIBANA, M
CDK5是已知在成熟脑中高度表达的cdc2相关蛋白激酶。本研究通过筛选成年小鼠cDNA文库，获得了小鼠CDK5 cDNA。北方杂交结果表明，小鼠CDK 5 mRNA在脑中表达量最高，在肾脏、睾丸和卵巢中表达量适中。在脑中，CDK5的表达在胚胎12.5天（E12.5）时已经观察到，并且其在整个胚胎阶段逐渐增加。出生后，该表达维持在高水平直至成年。原位杂交结果显示，CDK5 mRNA在脑、脊髓和周围神经节均有表达，尤其在海马锥体细胞、小脑浦肯野细胞、皮质神经元、嗅僧帽细胞、三叉神经中脑核和三叉神经运动核以及三叉神经节中表达最多。在任何部分中，在胶质细胞中均未观察到明显的表达。在胚胎发育过程中，CDK5在胚胎12.5天时已在三叉神经节和背根神经节中表达，在中枢神经系统中表达较强。CDK5的表达模式与CDC2的表达模式完全相反。CDK5在终末分化的非分裂神经元中表达的事实预示了除了控制细胞周期之外的替代功能。
CDK5 is a cdc2-related protein kinase that is known to be highly expressed in mature brain. In this study, we obtained a mouse CDK5 cDNA by screening an adult mouse cDNA library. Northern blot analysis demonstrated that the mouse CDK5 mRNA was expressed especially highly in brain, and moderately in kidney, testis and ovary. In brain the expression of CDK5 is already seen at embryonal 12.5 days (E12.5), and it gradually increases through the embryonal stage. After birth, the expression is maintained at a high level to adulthood. In situ hybridization demonstrated that the expression of CDK5 mRNA was distributed in neurons throughout the brain, spinal cord and peripheral ganglia, especially in the hippocampal pyramidal cells, cerebellar Purkinje cells, cortical neurons, olfactory mitral cells, mesencephalic and motor trigeminal nuclei and trigeminal ganglion. In any portion, no apparent expression was observed in glia. During development, the expression of CDK5 was already seen at E12.5 intensely in trigeminal and dorsal root ganglia, and moderately and diffusely in the central nervous system. The expression pattern of CDK5 is quite in contrast with that of CDC2. The fact that CDK5 is expressed in terminally differentiated non-dividing neurons predicts an alternative function(s) in addition to controlling the cell cycle.