The rudimentary locus of Drosophila melanogaster.
The rudimentary locus of Drosophila melanogaster.
复制标题
果蝇的基本基因座。
DOI：
10.1016/0022-2836(84)90441-8
复制
发表时间：
1984
影响因子：
5.6
通讯作者：
Jarry,BP
中科院分区：
文献类型：
--
作者：
Segraves,WA;Louis,C;Tsubota,S;Schedl,P;Rawls,JM;Jarry,BP
在本文报道的研究中，我们研究了黑腹果蝇（Drosophilamelanogaster）的营养基因的分子结构。初步编码一个220，000Mr多肽，催化嘧啶生物合成的前三个步骤：氨甲酰磷酸合成酶、天冬氨酸转氨甲酰酶和二氢乳清酸酶。我们已经确定了相对于基因座遗传图谱的基因转录方向。5′端映射到基因座的远端（相对于着丝粒），转录通过编码二氢乳清酸酶、氨甲酰磷酸合成酶和最后天冬氨酸转氨甲酰酶的结构域进行。该基因的起始转录单位长度为13.2 × 103bp，编码7.3 × 103bp的成熟信使RNA。其中一个插入序列长度超过4 × 103bp。最后，我们比较了果蝇rudimentary基因与酵母和仓鼠相应位点的DNA序列结构。
In the studies reported here, we have examined the molecular organization of therudimentarygene ofDrosophila melanogaster. rudimentaryencodes a 220,000Mr polypeptide catalyzing the first three steps in pyrimidine biosynthesis: carbamyl phosphate synthetase, aspartate transcarbamylase and dihydroorotase. We have determined the direction of transcription of the gene relative to the genetic map of the locus. The 5′ end maps to the distal end of the locus (relative to the centromere) and transcription proceeds through the domains encoding dihydroorotase, carbamyl phosphate synthetase and finally aspartate transcarbamylase. Therudimentarytranscription unit spans a DNA segment of 13.2 × 103base-pairs and encodes a mature messenger RNA of 7.3 × 103base-pairs. Three intervening sequences have been identified, one of which is over 4 × 103base-pairs in length. Finally, we have compared the DNA sequence organization of theDrosophila rudimentarygene with the corresponding loci of yeast and hamster.