MicroRNA-744 inhibited cervical cancer growth and progression through apoptosis induction by regulating Bcl-2
MicroRNA-744 inhibited cervical cancer growth and progression through apoptosis induction by regulating Bcl-2
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DOI：
10.1016/j.biopha.2016.04.023
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发表时间：
2016-07-01
影响因子：
7.5
通讯作者：
Liu, Yun
中科院分区：
文献类型：
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作者：
Chen, Xiao-Fang;Liu, Yun
越来越多的证据表明，microRNA在许多肿瘤进展（包括宫颈癌）的发展和转移中起着至关重要的作用。miR-744在多种肿瘤生长过程中存在异常表达，miR-744抑制宫颈癌细胞增殖和转移的机制尚不清楚。越来越多的证据表明Bcl-2信号通路在细胞凋亡、细胞生长和增殖等细胞过程中发挥重要作用。本研究的目的是筛选能够通过靶向Bcl-2表达抑制胃癌生长、迁移、侵袭、增殖和转移的miR-744。使用实时PCR（RT-qPCR）在体外和体内实验中定量miR-744的表达。通过细胞增殖测定miR-744的生物学功能。我们的研究表明，miR-744靶向Bcl-2，导致宫颈癌细胞凋亡信号的失活和细胞增殖，改善宫颈癌的生长和进展。此外，上调miR-744和下调Bcl-2均能刺激Caspase-3表达，促进宫颈癌细胞凋亡。因此，我们的研究揭示了miR-744和Bcl-2在宫颈癌发病机制中的机制联系，通过调节Caspase-3，导致宫颈癌细胞生长的抑制。靶向miR-744可能成为未来宫颈癌临床治疗的新策略。(C)2016 Elsevier Masson SAS。All rights reserved.
Growing evidence suggests that microRNA plays an essential role in the development and metastasis of many tumor progressions, including cervical cancer. Aberrant miR-744 expression has been indicated in many growth of tumor, the mechanism of miR-744 inhibits both the proliferation and metastatic ability for cervical cancer remains unclear. Accumulating evidences reported that Bcl-2 signal pathway plays an important role in the cellular process, such as apoptosis, cell growth and proliferation. The goal of this study was to identify miR-744 that could inhibit the growth, migration, invasion, proliferation and metastasis of gastric cancer through targeting Bcl-2 expression. Real-time PCR (RT-qPCR) was used to quantify miR-744 expression in vitro and vivo experiments. The biological functions of miR-744 were determined via cell proliferation. Our study indicated that miR-744 targeted on Bcl-2, which leads to the inactivation of apoptosis signaling and the cell proliferation of cervical cancer cells, ameliorating cervical cancer growth and progression. In addition, both up-regulation of miR-744 and down-regulation of Bcl-2 could stimulate Caspase-3 expression, promoting apoptosis of cervical cancer cells. Therefore, our research revealed the mechanistic links between miR-744 and Bcl-2 in the pathogenesis of cervical cancer through modulation of Caspase-3, leading to the inhibition of cervical cancer cell growth. And targeting miR-744 could be served as a novel strategy for future cervical cancer therapy clinically. (C) 2016 Elsevier Masson SAS. All rights reserved.