A receptor-like protein from Nicotiana benthamiana mediates VmE02 PAMP-triggered immunity
A receptor-like protein from Nicotiana benthamiana mediates VmE02 PAMP-triggered immunity
复制标题
来自本塞姆氏烟草的受体样蛋白介导 VmE02 PAMP 触发的免疫
DOI：
10.1111/nph.16995
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发表时间：
2020-11-04
期刊：
影响因子：
9.4
通讯作者：
Huang, Lili
中科院分区：
文献类型：
--
作者：
Nie, Jiajun;Zhou, Wenjing;Huang, Lili
植物利用它们的先天免疫系统来防御植物病原体。作为其中的一部分，模式触发免疫通过模式识别受体（PRR）检测病原体相关分子模式（PAMP）来激活。虽然越来越多的PAMPs已被鉴定，但其识别的PRRs仍然很大程度上未知。（对VmE 02的响应），其介导VmE 02的感知，VmE 02是先前使用病毒诱导的基因沉默（VIGS）、免疫共沉淀、我们发现RE 02的沉默显著减弱了VmE 02-介导的红细胞死亡和免疫应答。RE 02在体内和体外与VmE 02特异性相互作用，并且其对VmE 02显示出高亲和力。与受体样激酶SOBIR 1和BAK 1形成复合物是RE 02感知VmE 02所必需的。此外，RE 02沉默的植物表现出增强的敏感性，无论是卵菌辣椒疫霉和真菌核盘菌，而RE 02的过表达增加植物对这些病原体的抗性，我们的研究结果表明，PAMP VmE 02和受体样蛋白RE 02代表了一个新的配体-受体对植物免疫，RE 02代表了一个有前途的目标工程抗病性。
Plants use their innate immune system to defend against phytopathogens. As a part of this, pattern triggered-immunity is activated via pattern recognition receptor (PRR) detection of pathogen-associated molecular patterns (PAMPs). Although an increasing number of PAMPs have been identified, the PRRs for their recognition remain largely unknown.In the present study, we report a receptor-like protein RE02 (Response to VmE02) in Nicotiana benthamiana, which mediates the perception of VmE02, a PAMP previously identified from the phytopathogenic fungus Valsa mali, using virus-induced gene silencing (VIGS), co-immunoprecipitation, pull-down and microscale thermophoresis assays.We show that silencing of RE02 markedly attenuated VmE02-triggred cell death and immune responses. RE02 specifically interacted with VmE02 in vivo and in vitro, and it displayed a high affinity for VmE02. Formation of a complex with the receptor-like kinases SOBIR1 and BAK1 was essential for RE02 to perceive VmE02. Moreover, RE02-silenced plants exhibited enhanced susceptibility to both the oomycete Phytophthora capsici and the fungus Sclerotinia sclerotiorum, while overexpression of RE02 increased plant resistance to these pathogens.Together, our results indicate that the PAMP VmE02 and the receptor-like protein RE02 represent a new ligand-receptor pair in plant immunity, and that RE02 represents a promising target for engineering disease resistance.