Plasma Metabolic Profiles in Women are Menopause Dependent.
Plasma Metabolic Profiles in Women are Menopause Dependent.
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女性血浆代谢特征与更年期相关
DOI：
10.1371/journal.pone.0141743
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Li K
中科院分区：
文献类型：
--
作者：
Ke C;Hou Y;Zhang H;Yang K;Wang J;Guo B;Zhang F;Li H;Zhou X;Li Y;Li K
更年期是一种内分泌的转变，极大地影响了中老年妇女的健康和疾病易感性。为了获得新的见解更年期的代谢过程中，血浆代谢谱在115个绝经前和绝经后的妇女进行了系统的分析，超高效液相色谱/质谱结合单变量和多变量统计分析。代谢特征显示绝经前和绝经后妇女之间存在相当大的差异，在偏最小二乘判别分析评分图中观察到组间存在明显分离。总共有28种代谢物被确定为绝经期的潜在代谢物标志物，包括上调的酰基肉毒碱、脂肪酸、溶血磷脂酰胆碱、溶血磷脂酰乙醇胺和下调的孕甾烷-3-葡糖苷酸、硫酸脱氢表雄酮、对羟基苯乙酸和二氢硫辛酸。这些差异突出表明，绝经后妇女的脂肪酸β-氧化、磷脂代谢、激素代谢和氨基酸代谢发生了显著变化。总之，我们的血浆代谢组学研究提供了新的理解与绝经相关的代谢谱，并将有助于调查绝经相关疾病和评估代谢组学混杂因素。
Menopause is an endocrinological transition that greatly affects health and disease susceptibility in middle-aged and elderly women. To gain new insights into the metabolic process of menopause, plasma metabolic profiles in 115 pre- and post-menopausal women were systematically analyzed by ultra-performance liquid chromatography/mass spectrometry in conjunction with univariate and multivariate statistical analysis. Metabolic signatures revealed considerable differences between pre- and post-menopausal women, and clear separations were observed between the groups in partial least-squares discriminant analysis score plots. In total, 28 metabolites were identified as potential metabolite markers for menopause, including up-regulated acylcarnitines, fatty acids, lysophosphatidylcholines, lysophosphatidylethanolamines, and down-regulated pregnanediol-3-glucuronide, dehydroepiandrosterone sulfate, p-hydroxyphenylacetic acid and dihydrolipoic acid. These differences highlight that significant alterations occur in fatty acid β-oxidation, phospholipid metabolism, hormone metabolism and amino acid metabolism in post-menopausal women. In conclusion, our plasma metabolomics study provides novel understanding of the metabolic profiles related to menopause, and will be useful for investigating menopause-related diseases and assessing metabolomic confounding factors.