Segregation and Linkage of Several Genes in Cucumber
Segregation and Linkage of Several Genes in Cucumber
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DOI：
10.21273/jashs.126.4.442
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发表时间：
2001-07
影响因子：
1.9
通讯作者：
S. Walters;Nischit V. Shetty;T. C. Wehner
中科院分区：
文献类型：
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作者：
S. Walters;Nischit V. Shetty;T. C. Wehner
附加索引词。黄瓜，蔬菜育种，抗病性，基因突变体 摘要。使用黄瓜 (Cucumis sativus L.) 中的 18 个基因对 11 个科的基因连锁进行了研究。研究的基因是B（黑刺）、B-3（黑刺3）、B-4（黑刺4）、bi（无苦子叶）、Bt（苦果）、Bt-2（苦果-2）、D（暗果皮）、df（延迟开花）、de（决定性习性）、F（雌性表达）、gl（无毛叶子）、lh（长下胚轴）、ns （许多刺）、pm-h（下胚轴上表达的白粉病（Sphaerotheca fuliginea Schlecht.：Fr.）抗性）、ss（小刺）、Tu（结核果）、u（均匀的未成熟果实颜色）和 w（白色未成熟果实颜色）。本研究的一个主要目的是测量水果苦味基因（Bt 和 Bt-2）和刺颜色基因（B-3 和 B-4）与之前研究的基因座的联系：B、bi、D、de、df、F、gl、lh、ns、pm-h、ss、Tu、u 和 w。 LJ 90430 x PI 173889 的 F2 后代分离出 13 个苦味果实：3 个非苦味果实，表明不同的基因在这些品系中控制果实苦味。 Bt-2 被认为是 LJ 90430 中控制水果苦味的基因。它是与导致 PI 173889 中水果苦味的 Bt 不同的基因座。发现了几个新的基因连锁：bi-Bt、(Bt-2)-de、D-(Bt-2)、D-ns、gl-F、ss-(Bt-2)、Tu-(Bt-2) 和u—(Bt-2)。 Bt 基因似乎与 bi 连锁，并且可能位于连锁群 I 上。Bt-2 似乎与可以连接连锁群 I 和 IV 的几个基因连锁。 Bt-2 与 u、Tu、D 和 ss 连接，它们都属于连锁群 IV。还发现 Bt-2 与 de 松散连锁，即连锁群 I。在 B-3 和 B-4 以及本研究中评估的基因之间未发现连锁。几个基因组合（(Bt-2)-de、de—ns、de—ss、de—Tu、de—u、ns—F 和 ss—F）之间的弱连锁（>25 cM）提供了更多证据表明连锁组 I 和 IV 可能存在关联。由于联系较弱，需要使用更大的人群和更多的标记来获得更多信息来证实这些发现。
ADDITIONAL INDEX WORDS. Cucumis sativus, vegetable breeding, disease resistance, gene mutants ABSTRACT. Gene linkage was investigated in 11 families using 18 genes in cucumber (Cucumis sativus L.). The genes studied were B (black spine), B-3 (Black spine-3), B-4 (Black spine-4), bi (bitterfree cotyledons), Bt (bitter fruit), Bt-2 (bitter fruit- 2), D (dull fruit skin), df (delayed flowering), de (determinate habit), F (female sex expression), gl (glabrous foliage), lh (long hypocotyl), ns (numerous spines), pm-h (powdery mildew (Sphaerotheca fuliginea Schlecht.:Fr.) resistance expressed on the hypocotyl), ss (small spines), Tu (tuberculate fruit), u (uniform immature fruit color), and w (white immature fruit color). A major objective of this study was to measure linkages of genes for fruit bitterness (Bt and Bt- 2), and spine color (B-3 and B-4) relative to previously studied loci: B, bi, D, de, df, F, gl, lh, ns, pm-h, ss, Tu, u, and w. The F2 progeny of LJ 90430 x PI 173889 segregated 13 bitter fruit : 3 nonbitter fruit, indicating that different genes are controlling fruit bitterness in these lines. Bt-2 is proposed as the gene controlling bitterness of fruit in LJ 90430. It is a separate locus from Bt, that causes bitter fruit in PI 173889. Several new gene linkages were found: bi—Bt, (Bt-2)—de, D—(Bt-2), D—ns, gl—F, ss—(Bt-2), Tu—(Bt-2), and u—(Bt-2). The Bt gene appears to be linked to bi and may be located on linkage group I. Bt-2 appears to be linked with several genes that could connect linkage groups I and IV. Bt-2 was linked to u, Tu, D, and ss, that are all on linkage group IV. Bt-2 was also found to be linked loosely to de, that is on linkage group I. No linkages were found between B-3 and B-4 and the genes evaluated in this study. Weak linkages (>25 cM) between several gene combinations ((Bt-2)-de, de—ns, de—ss, de—Tu, de—u, ns—F, and ss—F) provided more evidence that linkage group I and IV may be linked. Due to the weak linkages, more information needs to be obtained using larger populations and more markers to confirm these findings.