Susceptibility of ferrets (Mustela putorius furo) to experimentally induced rabies with European Bat Lyssaviruses (EBLV)
Susceptibility of ferrets (Mustela putorius furo) to experimentally induced rabies with European Bat Lyssaviruses (EBLV)
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DOI：
10.1111/j.1439-0450.2004.00730.x
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发表时间：
2004-03-01
期刊：
影响因子：
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通讯作者：
Fooks, AR
中科院分区：
文献类型：
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作者：
Vos, A;Müller, T;Fooks, AR
对20只雪貂(Mustela Putorius Furo)进行肌肉接种。使用10(4.0)个病灶形成单位(FFU)EBLV-2(n=6)、10(4.0)个FFU EBLV-1(n=7)和10(6.0)FFU EBLV-1(n=7)注射欧洲BAT溶血病毒(EBLV)1型和2型。此外，15只小鼠接受10(2.5)FFU EBLV-2(n=5)、10(2.5)FFU EBLV-1(n=5)和10(4.5)FFU EBLV-1(n=5)肌肉注射。接种疫苗。所有接受高剂量EBLV-1的雪貂和小鼠都死于感染。相比之下，实验接种了较低剂量EBLV-1的七只雪貂和五只小鼠中只有三只死亡。相比之下，所有感染EBLV-2的雪貂和五只小鼠中的四只都存活了下来。所有20只受感染的雪貂都是血清转换。使用灵敏的分子工具，在不同的组织中检测到了该病毒，但在84天的观察期内采集的任何唾液样本中都没有发现该病毒。
Twenty ferrets (Mustela putorius furo) were inoculated by intramuscular (i.m.) injection with European Bat Lyssaviruses (EBLV) type-1 and 2 using 10(4.0) foci-forming units (FFU) EBLV-2 (n = 6) 10(4.0) FFU EBLV-1 (n = 7) and 10(6.0) FFU EBLV-1 (n = 7). Furthermore, 15 mice received 10(2.5) FFU EBLV-2 (n = 5) 10(2.5) FFU EBLV-1 (n = 5) and 10(4.5) FFU EBLV-1 (n = 5) by i.m. inoculation. All ferrets and mice receiving the hi-her dose of EBLV-1 succumbed to infection. In contrast, only three of seven ferrets and two of five mice inoculated experimentally with the lower EBLV-1 dose died. By comparison, all of the EBLV-2 infected ferrets and four of five mice survived infection. All 20 infected ferrets seroconverted. Using sensitive molecular tools, the virus was detected in different tissues, but it could not be found in any saliva samples taken during the 84-day observation period.