Near-Infrared IIb Fluorescence Imaging of Vascular Regeneration with Dynamic Tissue Perfusion Measurement and High Spatial Resolution
Near-Infrared IIb Fluorescence Imaging of Vascular Regeneration with Dynamic Tissue Perfusion Measurement and High Spatial Resolution
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DOI：
10.1002/adfm.201803417
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发表时间：
2018-09-05
影响因子：
19
通讯作者：
Huang, Ngan F.
中科院分区：
文献类型：
--
作者：
Ma, Zhuoran;Zhang, Mingxi;Huang, Ngan F.
实时光学成像是观察实验性外周动脉疾病（PAD）小鼠体内血流动力学和血管结构的一种很有前途的方法。本文报道了一种新的基于荧光的全光学成像方法在近红外IIb （NIR-IIb， 1500-1700 nm发射）窗口中的应用，用于对PAD模型小鼠后肢微血管和血液灌注进行成像。在模拟研究中，与单壁纳米管（SWNT） NIR-IIa （1000-1400 nm）染料相比，在不同的穿透深度下，硫化铅/硫化镉（PbS/CdS）量子点显示出更好的图像清晰度保持。当全身注射到小鼠体内时，与swnt相比，PbS/CdS显示出更高的血管清晰度，并且比体内显微镜计算机断层扫描具有更高的空间分辨率。在PAD小鼠模型中，NIR-IIb成像显示缺血后肢血管在10天内血流灌注显著改善（P < 0.05），并且在PAD诱导后的前7天微血管密度显著增加。总之，NIR-IIb成像PbS/CdS血管造影剂是一种有用的多功能成像方法，可用于微血管的高空间分辨率成像和血液灌注恢复的定量。
Real-time optical imaging is a promising approach for visualizing in vivo hemodynamics and vascular structure in mice with experimentally induced peripheral arterial disease (PAD). The application of a novel fluorescence-based all-optical imaging approach in the near-infrared IIb (NIR-IIb, 1500-1700 nm emission) window, for imaging hindlimb microvasculature and blood perfusion in a mouse model of PAD is reported. In phantom studies, lead sulfide/cadmium sulfide (PbS/CdS) quantum dots show better retention of image clarity, in comparison with single-walled nanotube (SWNT) NIR-IIa (1000-1400 nm) dye, at varying depths of penetration. When systemically injected to mice, PbS/CdS demonstrates improved clarity of the vasculature, compared to SWNTs, as well as higher spatial resolution than in vivo microscopic computed tomography. In a mouse model of PAD, NIR-IIb imaging of the ischemic hindlimb vasculature shows significant improvement in blood perfusion over the course of 10 days (P < 0.05), as well as a significant increase in microvascular density over the first 7 days after induction of PAD. In conclusion, NIR-IIb imaging of PbS/CdS vascular contrast agent is a useful multifunctional imaging approach for high spatial resolution imaging of the microvasculature and quantification of blood perfusion recovery.