SIALIDASES (NEURAMINIDASES) IN BACTERIAL VAGINOSIS AND BACTERIAL VAGINOSIS-ASSOCIATED MICROFLORA
SIALIDASES (NEURAMINIDASES) IN BACTERIAL VAGINOSIS AND BACTERIAL VAGINOSIS-ASSOCIATED MICROFLORA
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DOI：
10.1128/jcm.30.3.663-666.1992
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发表时间：
1992-03-01
影响因子：
9.4
通讯作者：
HILLIER, SL
中科院分区：
文献类型：
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作者：
BRISELDEN, AM;MONCLA, BJ;HILLIER, SL
细菌性阴道病、普雷沃菌属和拟杆菌属与早产和上生殖道感染有关。 普雷沃氏菌（拟杆菌）和脆弱拟杆菌也与早产有关。 然而，下生殖道感染导致上生殖道疾病的机制仍然知之甚少。 唾液酸酶（神经氨酸酶）是增强微生物侵入和破坏组织的能力的酶。 50例细菌性阴道病患者阴道液中42例（84%）唾液酸酶活性升高，而19例非细菌性阴道病患者阴道液中无唾液酸酶活性升高（P < 0.001）。 细菌性阴道病患者阴道液唾液酸酶的中位比活性为9.8 U，而非细菌性阴道病患者为2.5 U（P < 0.001）。 为了确定阴道液中唾液酸酶的可能来源，对治疗前后从患有细菌性阴道病的妇女中回收的微生物进行了测定。 在28例细菌性阴道病妇女的标本中，27例（96%）产生唾液酸酶阳性细菌，阴道液中位浓度为10（6.5）CFU/ml。 普雷沃菌属和拟杆菌属占26个阴道液中的唾液酸酶活性，而阴道加德纳菌占剩余液体中的唾液酸酶活性。 治疗后，在22名对治疗有反应的妇女中的1名（5%）和所有6名治疗失败的妇女的阴道液中检测到唾液酸酶。 这些数据表明，阴道液唾液酸酶与细菌性阴道病高度相关，这种酶活性的可能来源是阴道中存在的拟杆菌属和普雷沃氏菌属。
Bacterial vaginosis, Prevotella species, and Bacteroides species have been associated with prematurity and upper genital tract infection. Prevotella (Bacteroides) species and Bacteroides fragilis have also been associated with preterm birth. However, the mechanism by which lower genital tract infection causes upper genital tract disease remains poorly understood. Sialidases (neuraminidases) are enzymes which enhance the ability of microorganisms to invade and destroy tissue. Elevated levels of sialidase activity were detected in 42 (84%) of 50 vaginal fluid specimens from women with bacterial vaginosis and none of 19 vaginal fluids from women without bacterial vaginosis (P < 0.001). Vaginal fluid from women with bacterial vaginosis had a median specific activity of 9.8 U compared to 2.5 U of sialidase in women without bacterial vaginosis (P < 0.001). In order to determine the probable source of sialidases in vaginal fluid, the microorganisms recovered from women with bacterial vaginosis before and after treatment were assayed. Of 28 specimens from women with bacterial vaginosis, 27 (96%) yielded sialidase-positive bacteria, at a median concentration of 10(6.5) CFU/ml of vaginal fluid. Prevotella and Bacteroides species accounted for the sialidase activity in 26 of the vaginal fluids, and Gardnerella vaginalis accounted for the sialidase activity in the remaining fluid. After treatment, sialidase was detected in the vaginal fluid of 1 (5%) of 22 women who responded to therapy and in all of 6 women for whom therapy failed. These data suggest that vaginal fluid sialidase is highly correlated with bacterial vaginosis and that the probable sources for this enzyme activity are the Bacteroides and Prevotella species present in the vagina.