Seasonal differences of gene expression profiles in song sparrow (Melospiza melodia) hypothalamus in relation to territorial aggression.
Seasonal differences of gene expression profiles in song sparrow (Melospiza melodia) hypothalamus in relation to territorial aggression.
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DOI：
10.1371/journal.pone.0008182
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发表时间：
2009-12-04
期刊：
影响因子：
3.7
通讯作者：
Wingfield JC
中科院分区：
文献类型：
--
作者：
Mukai M;Replogle K;Drnevich J;Wang G;Wacker D;Band M;Clayton DF;Wingfield JC
雄性歌麻雀（Melospiza melodia）领土全年，然而，神经内分泌反应模拟领土入侵（STI）不同的繁殖季节（春季）和非繁殖季节（秋季）。在春季，暴露于性传播感染会导致黄体生成素和睾丸激素的增加，但在秋季则不然。这些观察结果表明，在不同季节之间，驱动神经内分泌对STI反应的机制存在根本差异。利用基因芯片技术，研究了斑胸草雀在两个不同季节进行领土攻击后下丘脑基因表达谱的变化。自由生活的领土男性歌麻雀暴露于同种或异种（对照）男性在春季和秋季的STI。记录行为数据，收集整个下丘脑，并进行微阵列杂交。进行定量PCR以进行验证。结果表明，262个cDNA在春季和秋季之间在对照鸟类中差异表达。在秋季，有173个cDNA受到STI的显著影响;而在春季，只有67个cDNA受到STI的显著影响。有88个cDNA在季节和STI中表现出显著的相互作用。结果表明，STI驱动下丘脑在春季与秋季的差异基因组反应。与季节有关的差异表达的cDNA的数量大于与社会互动有关的，这表明下丘脑中的主要潜在季节性影响可能决定了社会互动后的差异反应。功能途径分析表明，调节甲状腺激素作用和神经可塑性的基因是这种神经内分泌调节的靶点。
Male song sparrows (Melospiza melodia) are territorial year-round; however, neuroendocrine responses to simulated territorial intrusion (STI) differ between breeding (spring) and non-breeding seasons (autumn). In spring, exposure to STI leads to increases in luteinizing hormone and testosterone, but not in autumn. These observations suggest that there are fundamental differences in the mechanisms driving neuroendocrine responses to STI between seasons. Microarrays, spotted with EST cDNA clones of zebra finch, were used to explore gene expression profiles in the hypothalamus after territorial aggression in two different seasons. Free-living territorial male song sparrows were exposed to either conspecific or heterospecific (control) males in an STI in spring and autumn. Behavioral data were recorded, whole hypothalami were collected, and microarray hybridizations were performed. Quantitative PCR was performed for validation. Our results show 262 cDNAs were differentially expressed between spring and autumn in the control birds. There were 173 cDNAs significantly affected by STI in autumn; however, only 67 were significantly affected by STI in spring. There were 88 cDNAs that showed significant interactions in both season and STI. Results suggest that STI drives differential genomic responses in the hypothalamus in the spring vs. autumn. The number of cDNAs differentially expressed in relation to season was greater than in relation to social interactions, suggesting major underlying seasonal effects in the hypothalamus which may determine the differential response upon social interaction. Functional pathway analyses implicated genes that regulate thyroid hormone action and neuroplasticity as targets of this neuroendocrine regulation.
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