Role of polyamines in peach fruit development and storage
Role of polyamines in peach fruit development and storage
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DOI：
10.1093/treephys/26.6.791
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发表时间：
2006-06-01
期刊：
影响因子：
4
通讯作者：
Moriguchi, Takaya
中科院分区：
文献类型：
--
作者：
Liu, Jihong;Nada, Kazuyoshi;Moriguchi, Takaya
为了研究多胺在“Akatsuki”桃（Prunus persica (L.) Batsch.）采收前和收获后果实发育中的作用，我们测量了多胺浓度、多胺生物合成酶的活性以及编码这些酶的基因的表达。收获前果实中游离多胺、腐胺 (Put)、亚精胺 (Spd) 和精胺 (Spm) 的浓度在盛花 (DAF) 后 16 天达到峰值，然后逐渐下降直至收获，但 Put 除外，它在 94 DAF 处出现第二个峰值，就在乙烯产生开始之前。在收获后的果实中，观察到 Spd 和 Spin 浓度的微小变化，而 Put 浓度在收获当天达到峰值，随后突然下降，随后增加 2 倍，这与乙烯产量的波动模式相反。精氨酸脱羧酶(ADC)和鸟氨酸脱羧酶(ODC)活性在果实发育初期达到峰值，然后下降，直到80 DAF，之后活性低于检测限，表明Put是在果实发育早期合成的。 S-腺苷甲硫氨酸脱羧酶(SAM-DC)的活性逐渐下降直至S2结束。采前和采后果实中五种假定的多胺生物合成基因（ADC、ODC、SAMDC、亚精胺合酶（SPDS）和精胺合酶（SPMS））的表达水平与观察到的酶活性和多胺浓度的变化并不完全一致。讨论了多胺在桃果实发育过程中的可能作用以及多胺与乙烯生物合成之间的关系。
To investigate the role of polyamines in pre- and post-harvest fruit development of 'Akatsuki' peach (Prunus persica (L.) Batsch.) we measured polyamine concentrations, activities of polyamine biosynthetic enzymes and expression of genes encoding these enzymes. Concentrations of the free polyamines, putrescine (Put), spermidine (Spd) and spermine (Spm) in pre-harvest fruit peaked 16 days after full bloom (DAF) and then progressively decreased until harvest with the exception of Put, which showed a second peak at 94 DAF, just before the onset of ethylene production. In post-harvest fruit, minor changes in concentrations of Spd and Spin were observed, whereas Put concentration peaked on the harvest day, followed by an abrupt decrease and a subsequent 2-fold increase, which was opposite to the fluctuating pattern of ethylene production. Activities of arginine decarboxylase (ADC) and ornithine decarboxylase (ODC) peaked during the first stage of fruit development and then decreased until 80 DAF, after which the activities were below detection limits, suggesting that Put is synthesized during the early stage of fruit development. Activity of S-adenosylmethionine decarboxylase (SAM-DC) decreased progressively until the end of S2. Expression levels of five putative polyamine biosynthetic genes, ADC, ODC, SAMDC, spermidine synthase (SPDS) and spermine synthase (SPMS), in pre-harvest and post-harvest fruit did not coincide precisely with the observed changes in enzymatic activities and polyamine concentrations. The possible role of polyamines during peach fruit development and the relationship between polyamines and ethylene biosynthesis are discussed.