The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.
The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.
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在高pH值中使用柠檬酸铅作为电子显微镜中的电子斑点。
DOI：
10.1083/jcb.17.1.208
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发表时间：
1963-04
影响因子：
7.8
通讯作者：
REYNOLDS, E S
中科院分区：
文献类型：
--
作者：
REYNOLDS, E S
用铅盐水溶液（1,2）和氢氧化铅饱和溶液(1)作为染色剂，增强电子显微镜下观察的生物材料组分的电子散射特性。饱和的氢氧化铅溶液(1)，虽然比醋酸铅或一碱性醋酸铅（1,2）的染色更强烈，但暴露在空气中形成不溶性碳酸铅。避免在染色过程中污染切片表面的这种沉淀物刺激了开发排除空气或二氧化碳的详细程序（3,4）。最近出现了几种沃森氢氧化铅染色法(1)的改进（5-7）。所有这些都使用相对较高的pH值（大约12），并且除了铅之外还含有少量酒石酸盐(6)，一种相对弱的络合剂(8)。这些改性的铅渍不太容易用沉淀的污渍产品污染截面表面。这里报告的染色不同于以前的碱性铅染色
Aqueous solutions of lead salts (1, 2) and saturated solutions of lead hydroxide (1) have been used as stains to enhance the electron-scattering properties of components of biological materials examined in the electron microscope. Saturated solutions of lead hydroxide (1), while staining more intensely than either lead acetate or monobasic lead acetate (l, 2), form insoluble lead carbonate upon exposure to air. The avoidance of such precipitates which contaminate surfaces of sections during staining has been the stimulus for the development of elaborate procedures for exclusion of air or carbon dioxide (3, 4).Several modifications of Watson's lead hydroxide stain (1) have recently appeared (5-7). All utilize relatively high pH (approximately 12) and one contains small amounts of tartrate (6), a relatively weak complexing agent (8), in addition to lead. These modified lead stains are less liable to contaminate the surface of the section with precipitated stain products. The stain reported here differs from previous alkaline lead stains in