Raw meat based diet influences faecal microbiome and end products of fermentation in healthy dogs
Raw meat based diet influences faecal microbiome and end products of fermentation in healthy dogs
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DOI：
10.1186/s12917-017-0981-z
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发表时间：
2017-02-28
影响因子：
2.6
通讯作者：
Stefanon, Bruno
中科院分区：
文献类型：
--
作者：
Sandri, Misa;Dal Monego, Simeone;Stefanon, Bruno
背景资料：为了更深入地了解不同饲养条件下健康犬肠道微生物生态系统的变异性，并改进饲料配方，需要进行膳食干预研究。这项研究的目的是调查在狗的影响，以原料为基础的饮食补充蔬菜食品对粪便微生物组比较挤压food.Methods：8个健康的成年拳击手犬被招募，并随机分为两个实验组的4个人。定期给犬饲喂市售挤压饲料（RD），从试验开始时开始，一组接受生食基础饲料（MD），另一组继续饲喂RD饲料（CD），持续两周。14天后，将两组颠倒，CD组转移到MD，MD转移到CD，持续14天。在研究开始时（T0）、改变饮食前14天后（T14）和实验期结束时（T28）收集粪便用于DNA提取和宏基因组分析，通过测序16 SrRNA V3和V4区、短链脂肪酸（SCFA）、乳酸和粪便评分。乳酸杆菌的比例降低，副乳属（P < 0.01）和普雷沃菌属MD组动物的属数显著增加（P < 0.05），Shannon生物多样性指数显著增加（P < 0.05）（3.31 +/- 0.15）与RD组（2.92 +/- 0.31; P < 0.05）相比。与RD组相比，MD组粪便中乳酸浓度显著升高（P <0.01），粪便评分显著降低（P <0.05）。粪乙酸与大肠埃希菌/志贺菌、巨单胞菌呈负相关（P < 0.01），而丁酸与布劳特氏菌、消化球菌呈正相关（P < 0.05）。乳酸与巨单胞菌属（P < 0.05）、大肠杆菌/志贺氏菌属（P < 0.01）和乳球菌属（P < 0.01）呈正相关。与RD饮食相比，MD饮食的施用促进了细菌群落的更平衡的生长和健康肠道功能读数的积极变化。
Background: Dietary intervention studies are required to deeper understand the variability of gut microbial ecosystem in healthy dogs under different feeding conditions and to improve diet formulations. The aim of the study was to investigate in dogs the influence of a raw based diet supplemented with vegetable foods on faecal microbiome in comparison with extruded food.Methods: Eight healthy adult Boxer dogs were recruited and randomly divided in two experimental blocks of 4 individuals. Dogs were regularly fed a commercial extruded diet (RD) and starting from the beginning of the trial, one group received the raw based diet (MD) and the other group continued to be fed with the RD diet (CD) for a fortnight. After 14 days, the two groups were inverted, the CD group shifted to the MD and the MD shifted to the CD, for the next 14 days. Faeces were collected at the beginning of the study (T0), after 14 days (T14) before the change of diet and at the end of experimental period (T28) for DNA extraction and analysis of metagenome by sequencing 16SrRNA V3 and V4 regions, short chain fatty acids (SCFA), lactate and faecal score.Results: A decreased proportion of Lactobacillus, Paralactobacillus (P < 0.01) and Prevotella (P < 0.05) genera was observed in the MD group while Shannon biodiversity Index significantly increased (3.31 +/- 0.15) in comparison to the RD group (2.92 +/- 0.31; P < 0.05). The MD diet significantly (P < 0.05) decreased the Faecal Score and increased the lactic acid concentration in the feces in comparison to the RD treatment (P < 0.01). Faecal acetate was negatively correlated with Escherichia/Shigella and Megamonas (P < 0.01), whilst butyrate was positively correlated with Blautia and Peptococcus (P < 0.05). Positive correlations were found between lactate and Megamonas (P < 0.05), Escherichia/Shigella (P < 0.01) and Lactococcus (P < 0.01).Conclusion: These results suggest that the diet composition modifies faecal microbial composition and end products of fermentation. The administration of MD diet promoted a more balanced growth of bacterial communities and a positive change in the readouts of healthy gut functions in comparison to RD diet.