Endothelial cell and hepatocyte deaths occur by apoptosis after ischemia-reperfusion injury in the rat liver
Endothelial cell and hepatocyte deaths occur by apoptosis after ischemia-reperfusion injury in the rat liver
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DOI：
10.1097/00007890-199904270-00003
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发表时间：
1999-04-27
期刊：
影响因子：
6.2
通讯作者：
Clavien, PA
中科院分区：
文献类型：
--
作者：
Kohli, V;Selzner, M;Clavien, PA
背景肝脏缺血性损伤通常被认为是导致坏死的原因，但最近人们认识到，在缺血/再灌注过程中，凋亡介质被激活。本研究旨在描述在缺血和再灌注的不同时期肝脏内发生凋亡的细胞的程度和类型。雄性Wistar大鼠进行30或60分钟的常温缺血，在缺血结束时和再灌注后1，6，24和72小时的肝切片进行评价，通过DNA片段化和凝胶电泳评估凋亡，原位染色凋亡细胞用TdT介导的dUTP-地高辛缺口末端标记（TUNEL），和形态学上的电子显微镜。肝活检标本原位TUNEL染色显示再灌注后有明显的细胞凋亡，肝窦内皮细胞（SEC）的凋亡早于肝细胞。例如，缺血60分钟后再灌注1小时，SEC染色的TUNEL阳性率为22+/-4%，而肝细胞染色的TUNEL阳性率为2+/-1%（P
Background Ischemic injury of the liver is generally considered to result in necrosis, but it has recently been recognized that mediators of apoptosis are activated during ischemia/reperfusion. This study was designed to characterize the extent and the type of cells within the liver that undergo apoptosis at different periods of ischemia and reperfusion,Methods. Male Wistar rats were subjected to 30 or 60 min of normothermic ischemia, Liver sections were evaluated at the end of ischemia and at 1, 6, 24, and 72 hr after reperfusion, Apoptosis was determined by DNA fragmentation as evaluated by laddering on gel electrophoresis, in situ staining for apoptotic cells using TdT-mediated dUTP-digoxigenin nick-end labeling (TUNEL), and morphology on electron microscopy,Results. In situ staining of liver biopsy specimens using TUNEL showed significant apoptosis after reperfusion, Sinusoidal endothelial cells (SEC) showed evidence of apoptosis earlier than hepatocytes, For example, at 1 hr of reperfusion after 60 min of ischemia, 22+/-4% of the SEC stained TUNEL positive compared with 2+/-1% of the hepatocytes (P