Simultaneous determination of flavonoid and alkaloid compounds in Citrus herbs by high-performance liquid chromatography - photodiode array detection - electro spray mass spectrometry
Simultaneous determination of flavonoid and alkaloid compounds in Citrus herbs by high-performance liquid chromatography - photodiode array detection - electro spray mass spectrometry
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高效液相色谱-光电二极管阵列检测-电喷雾质谱法同时测定柑橘类药材中的黄酮类和生物碱类化合物
DOI：
10.1016/j.jchromb.2007.07.018
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发表时间：
2007-10-01
影响因子：
3
通讯作者：
Yao, Shouzhuo
中科院分区：
文献类型：
--
作者：
Ding, Li;Luo, Xubiao;Yao, Shouzhuo
柑橘属植物中的主要活性成分是黄酮类化合物，尤其是橙皮苷、柚皮苷和生物碱类化合物，主要是黄酮类化合物，对人体健康具有有益的作用。它们可作为柑橘类药材质量控制的分子标记。本文提出了一种新的离子对色谱法，可将极性最强的溶质（synethyl）从溶液体积中排除，而保持另两种溶质（橙皮苷和柚皮苷）之间的选择性。全氟羧酸，这是适当的MS检测，由于其挥发性，被用作离子配对剂。采用全氟羧酸，成功地解决了共沸物分离过程中谱带拖尾和保留率低的问题。在所研究的三种全氟羧酸中，七氟丁酸（HFBA）的效果最好。对于黄酮苷类化合物，全氟酸对保留时间的影响很小。两种不同的分析物在一次运行中使用等度洗脱液分离令人满意，总的分析时间需要不到10分钟。使用选择离子监测（SIM）模式记录的伪分子离子的丰度为135.1，273.1和303.1，分别为syneseutine，柚皮苷和橙皮苷。用该方法同时测定了几种柑橘类药材中橙皮苷、柚皮苷和辛纳屈的含量。(c)2007 Elsevier B.V.保留所有权利。
The major active biological constituents in Citrus herbs are flavonoids, especially hesperidin, naringin and alkaloids, mainly synephrine, with beneficial medical effects on human health. They are used as the markers to control the quality of Citrus herbs. In this paper, a new ion pairing chromatographic method was developed to exclude the most polar solute (synephrine) from the viod volume and to maintain selectivity between the two other solutes (hesperidin and naringin). Perfluorinated carboxylic acids, which are appropriate for MS detection due to their volatility, were used as ion-pairing agents. The problems of the synephrine separation, such as band tailing and low retention, were solved successfully by using perfluorinated carboxylic acids. The effect of heptafluorobutyric acid (HFBA) was the best in the three investigated perfluorinated carboxylic acids. For the flavanone glycosides, the influence of the perfluorinated acids on retention time was rather weak. The two different kinds of the analytes were separated satisfactorily in one run using an isocratic eluent and the total analysis time takes less than 10 min. The abundance of pseudomolecular ions was recorded using selected ion monitoring (SIM) mode of m/z 135.1, 273.1 and 303.1 for synephrine, naringin and hesperidin, respectively. The contents of hesperidin, naringin and synephrine in several Citrus herbs were simultaneously determined by the proposed method. (c) 2007 Elsevier B.V. All rights reserved.