Monocyte adhesion to balloon-injured arteries: the influence of endothelial cell seeding.
Monocyte adhesion to balloon-injured arteries: the influence of endothelial cell seeding.
复制标题
单核细胞粘附到球囊损伤的动脉：内皮细胞接种的影响。
DOI：
10.1067/mva.2001.114211
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发表时间：
2001
影响因子：
4.3
通讯作者：
Conte,MS
中科院分区：
文献类型：
--
作者：
Gangadharan,SP;Eslami,MH;Weiss,IP;Sui,X;Conte,MS
动脉损伤的去内皮化破坏了正常的血管稳态，影响了血管壁的结构完整性，以及动脉表面与血液成分（如血小板、白细胞和循环蛋白）的相互作用。白细胞，特别是单核细胞向受损血管的募集与内膜增生的发病机制有关。我们假设，再内皮化是一个重要的调节剂单核细胞粘附愈合动脉surfaces.MethodsNew Zealand白色兔（n = 20）进行了双边髂股动脉球囊损伤。培养的自体静脉内皮细胞（EC）立即接种到一条血管上，而对侧动脉仅接受培养基，以加速内皮细胞的重新排列。血管被收获（5-9天后损伤）的渗透性（伊文思蓝染料排除），内皮覆盖（抗CD 31免疫组织化学），单核细胞粘附（离体结合51 Na 2CrO 4标记的单核细胞THP-1细胞），和单核细胞募集（RAM-11免疫组织化学）的分析。EC接种动脉的血管壁通透性显著降低（29% ± 10% vs 99% ± 0%表面伊文思蓝染色，P <0.005），与功能性内皮屏障的恢复一致。EC接种显著降低了离体THP-1与损伤后平均8天破裂的血管的结合（45，170 ± 8939 vs 85，994 ± 16，500个细胞/cm 2，P <0.05）。然而，RAM-11染色显示损伤后1周接种和对照血管之间的总体巨噬细胞积聚没有显著差异（111 ± 22 vs 95 ± 14个细胞/切片，P = 0.36）。在1周时，与损伤对照相比，屏障功能得到改善，并且接种的血管表面离体与活化的单核细胞的粘附性较低。尽管如此，EC接种和非接种动脉在1周内表现出相似的总巨噬细胞蓄积。这些数据表明，机械性动脉损伤后，内皮覆盖可能是影响白细胞粘附的一个重要变量。（J Vasc Surg 2001;33：1247-54.）
ObjectiveDeendothelialization of injuries of the artery disrupts normal vascular homeostasis, affecting both the structural integrity of the blood vessel wall, as well as the interaction of the arterial surface with blood components such as platelets, leukocytes, and circulating proteins. Leukocyte and, in particular, monocyte recruitment to damaged vessels has been implicated in the pathogenesis of intimal hyperplasia. We hypothesize that reendothelialization is an important modulator of monocyte adhesion to healing arterial surfaces.MethodsNew Zealand white rabbits (n = 20) were subjected to bilateral iliofemoral artery balloon injury. Cultured, autologous venous endothelial cells (ECs) were immediately seeded onto one vessel, whereas the contralateral artery received medium alone, to accelerate endothelial relining. Vessels were harvested (5-9 days after injury) for analysis of permeability (Evans Blue dye exclusion), endothelial coverage (anti-CD31 immunohistochemistry), monocyte adhesion (ex vivo binding of51Na2CrO4-labeled monocytic THP-1 cells), and monocyte recruitment (RAM-11 immunohistochemistry).ResultsImproved EC coverage was evidenced by positive staining for CD31 in the seeded vessels. Vessel wall permeability was markedly reduced in EC-seeded arteries (29% ± 10% vs 99% ± 0% surface Evans blue staining, P < .005), consistent with restoration of a functional endothelial barrier. EC seeding significantly reduced ex vivo THP-1 binding to vessels explanted at a mean of 8 days after injury (45,170 ± 8939 vs 85,994 ± 16,500 cells/cm2, P < .05). However, RAM-11 staining revealed no significant difference in overall macrophage accumulation between seeded and control vessels 1 week after injury (111 ± 22 vs 95 ± 14 cells/section, P = .36).ConclusionsImmediate seeding of a balloon-injured rabbit artery with cultured ECs results in accelerated restoration of the endothelial lining. At 1 week, barrier function is improved, and the seeded vessel surface is less adhesive to activated monocytes ex vivo, as compared with injured controls. Nonetheless, EC-seeded and nonseeded arteries demonstrate similar total macrophage accumulation over 1 week. These data suggest that after mechanical arterial injury, endothelial coverage may be one important variable influencing leukocyte adhesion. (J Vasc Surg 2001;33:1247-54.)