Regulation of NF-κB Signaling in Osteoclasts and Myeloid Progenitors
Regulation of NF-κB Signaling in Osteoclasts and Myeloid Progenitors
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DOI：
10.1007/978-1-4939-2422-6_31
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发表时间：
2015-01-01
期刊：
影响因子：
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通讯作者：
Abu-Amer, Yousef
中科院分区：
文献类型：
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作者：
Swarnkar, Gaurav;Abu-Amer, Yousef
活化B细胞的转录因子核因子kappa-轻链增强子（NF-kappa B）对免疫反应和骨骼发育至关重要。近年来的研究表明，NF-kappa B家族的各种成员都是调节骨细胞活性和存活以及骨代谢的可行靶点。在这方面，上游激酶或远端nf - κ B亚基的缺失导致骨畸形。因此，越来越明显的是，对骨细胞中nf - κ B的详细研究可能为设计新的治疗方式提供机会。在本章中，我们提出了改进的方法，描述了在体外和体内调节NF-kappa B通路的有效方法，以评估其在骨细胞和组织中的功能。
The transcription factor nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kappa B) is crucial for immune responses and skeletal development. Work in recent years has shown that various members of the NF-kappa B family are viable targets to regulate activity and survival of bone cells and hence bone metabolism. In this regard, deletion of upstream kinases or distal NF-kappa B subunits resulted with bone deformities. Thus, it has become increasingly apparent that detailed investigation of NF-kappa B in bone cells may provide opportunities to design new therapeutic modalities. In this chapter we present modified methodology describing efficient approaches to regulate the NF-kappa B pathway in vitro and in vivo to assess its function in bone cells and tissues.