Microfluidic System for Automated Cell-based Assays.
Microfluidic System for Automated Cell-based Assays.
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DOI：
10.1016/j.jala.2007.07.001
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发表时间：
2007-12-01
期刊：
影响因子：
--
通讯作者：
Hung, Paul J
中科院分区：
文献类型：
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作者：
Lee, Philip J;Ghorashian, Navid;Hung, Paul J
微流控细胞培养技术在药物筛选领域具有广阔的应用前景。主要优点包括改进的生物功能，更高质量的基于细胞的数据，减少试剂消耗和更低的成本。在这项工作中，我们展示了微流体细胞培养设计如何适应与标准96孔板格式兼容。关键的设计特点包括消除管道和连接器，使用被动重力驱动泵保持长期连续灌注细胞培养的能力，以及对微流控板出口井的直接分析。单个微流体培养板包含8个独立的流动单元，每个流动单元有10(4)个细胞，流速为50 μ l/天（停留时间6分钟）。使用商业乳酸脱氢酶（LDH）板读仪测定，在这种格式培养的HeLa细胞上测量抗癌药物依托泊苷的细胞毒性。微流控细胞培养方法与商业自动化能力的集成为改进基于细胞的筛选提供了一个令人兴奋的机会。
Microfluidic cell culture is a promising technology for applications in the drug screening industry. Key benefits include improved biological function, higher quality cell-based data, reduced reagent consumption, and lower cost. In this work, we demonstrate how a microfluidic cell culture design was adapted to be compatible with the standard 96-well plate format. Key design features include the elimination of tubing and connectors, the ability to maintain long term continuous perfusion cell culture using a passive gravity driven pump, and direct analysis on the outlet wells of the microfluidic plate. A single microfluidic culture plate contained 8 independent flow units, each with 10(4) cells at a flow rate of 50 mul/day (6 minute residence time). The cytotoxicity of the anti-cancer drug etoposide was measured on HeLa cells cultured in this format, using a commercial lactate dehydrogenase (LDH) plate reader assay. The integration of microfluidic cell culture methods with commercial automation capabilities offers an exciting opportunity for improved cell-based screening.