Effects of gamma-interferon on human neutrophils: protection from deterioration on storage.
Effects of gamma-interferon on human neutrophils: protection from deterioration on storage.
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DOI：
10.1182/blood.v80.1.225.225
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发表时间：
1992-07
期刊：
影响因子：
20.3
通讯作者：
S. Klebanoff;S. Olszowski;W. V. Van Voorhis;J. Ledbetter;AM Waltersdorph;KG Schlechte
中科院分区：
文献类型：
--
作者：
S. Klebanoff;S. Olszowski;W. V. Van Voorhis;J. Ledbetter;AM Waltersdorph;KG Schlechte
人多形核白细胞 (PMN) 与 100 U/mL γ-干扰素 (IFN-γ) 预培养过夜，对肿瘤坏死因子 (TNF)、粒细胞巨噬细胞集落刺激因子 (GM-CSF) 的刺激，通过碘化和/或超氧化物的产生来测量，代谢反应增强。 与在不存在 IFN-γ 的情况下预孵育的细胞相比，甲酰甲硫氨酰-亮氨酰-苯丙氨酸 (FMLP)、调理酵母聚糖和脂多糖 (LPS) 的含量更高。 IFN-γ 也部分阻止了储存的 PMN 杀葡萄球菌活性的下降，用佛波醇肉豆蔻酸酯乙酸酯 (PMA)、FMLP、TNF、GM-CSF 和 LPS 刺激的 PMN 粘附性降低也是如此。 IFN-γ 对 PMN 功能的这种保护作用与补体受体三 (CR3) α 链 (CD11b)、CR3 β 链 (CD18)、FcRII (CD32) 和 FcRIII (CD16) 的表面表达延长以及表面 FcRI (CD64) 的出现有关。聚合酶链式反应 (PCR) 用于扩增中性粒细胞 RNA 衍生的 cDNA，该 cDNA 可由根据已公布的特定蛋白质核苷酸序列设计的合成寡核苷酸识别。使用该程序，发现 gp91-phox、p67-phox、p47-phox、CD64、两种形式的 CD32、CD16、CD11b、CD18 和肌动蛋白的 mRNA 在中性粒细胞过夜储存后降低，并且这种稳态 mRNA 水平的降低部分或完全被 IFN-γ 阻止。 CD64 和 gp91-phox mRNA 通常会被 IFN-γ 增加至高于新鲜分离的中性粒细胞的水平。 CD64 和 p47 phox mRNA 的 Northern 分析证实了 PCR 方法的发现。这些发现表明，IFN-γ 的存在有利于将中性粒细胞储存在功能活跃的状态。
Human polymorphonuclear leukocytes (PMN) preincubated overnight with 100 U/mL gamma-interferon (IFN-gamma) had an increased metabolic response, as measured by iodination and/or superoxide production, to stimulation by tumor necrosis factor (TNF), granulocyte-macrophage colony-stimulating factor (GM-CSF), formylmethionyl-leucyl-phenylalanine (FMLP), opsonized zymosan, and lipopolysaccharide (LPS), as compared with cells comparably preincubated in the absence of IFN-gamma. The decline in the staphylocidal activity of the stored PMN was also prevented in part by IFN-gamma, as was the depressed adherence of PMN stimulated with phorbol myristate acetate (PMA), FMLP, TNF, GM-CSF, and LPS. This protective effect of IFN-gamma on PMN function was associated with the prolonged surface expression of the complement receptor three (CR3) alpha-chain (CD11b), CR3 beta-chain (CD18), FcRII (CD32), and FcRIII (CD16), and the appearance of surface FcRI (CD64). The polymerase chain reaction (PCR) was used to amplify neutrophil RNA-derived cDNA recognized by synthetic oliogonucleotides designed from published nucleotide sequences for specific proteins. Using this procedure, mRNA for gp91-phox, p67-phox, p47-phox, CD64, two forms of CD32, CD16, CD11b, CD18, and actin were found to be depressed after overnight storage of neutrophils, and this decrease in steady-state mRNA levels was in part or totally prevented by IFN-gamma. CD64 and gp91-phox mRNA were generally increased by IFN-gamma to a level greater than that of freshly isolated neutrophils. Northern analysis of CD64 and p47 phox mRNAs confirmed the findings with the PCR method. These findings suggest that storage of PMN in a functionally active state is favored by the presence of IFN-gamma.