FLI-1 Flightless-1 and LET-60 Ras control germ line morphogenesis in C. elegans.
FLI-1 Flightless-1 and LET-60 Ras control germ line morphogenesis in C. elegans.
复制标题
DOI：
10.1186/1471-213x-8-54
复制
发表时间：
2008-05-16
影响因子：
--
通讯作者：
Lundquist, Erik A.
中科院分区：
文献类型：
--
作者：
Lu, Jiamiao;Dentler, William L.;Lundquist, Erik A.
在秀丽隐杆线虫种系中，合胞体种系核在退出有丝分裂并进入减数分裂时排列在种系的皮层，形成种系细胞质的无核核心，称为轴。轴形成和种系组织的分子机制尚不清楚。 fli-1 基因突变会破坏轴组织，但不会影响减数分裂分化，这是秀丽隐杆线虫的一种表型，此处称为种系形态发生 (Glm) 表型。在fli-1突变体中，减数分裂胚核链跨越轴，并部分被胚系质膜内陷所包裹，类似于皮层的细胞核。围绕种系的体细胞鞘细胞的延伸伸入轴深处，并与 fli-1 突变体中的移位细胞核相关。 fli-1 编码具有富含亮氨酸重复序列和凝溶胶蛋白重复序列的分子，类似于果蝇 Flyless 1 和人类 Fliih，已被证明可以充当细胞质肌动蛋白调节剂以及核转录调节剂。 let-60 Ras 的突变（之前与种系发育有关）被发现会导致 Glm 表型。组成型活性的 LET-60 部分挽救了 fli-1 Glm 表型，表明 LET-60 Ras 和 FLI-1 可能共同作用来控制种系形态发生。 FLI-1 控制种系形态发生和轴组织，这一过程在分子水平上知之甚少。 LET-60 Ras GTPase 可能与 FLI-1 共同作用来控制种系形态发生。
In the C. elegans germ line, syncytial germ line nuclei are arranged at the cortex of the germ line as they exit mitosis and enter meiosis, forming a nucleus-free core of germ line cytoplasm called the rachis. Molecular mechanisms of rachis formation and germ line organization are not well understood. Mutations in the fli-1 gene disrupt rachis organization without affecting meiotic differentiation, a phenotype in C. elegans referred to here as the germ line morphogenesis (Glm) phenotype. In fli-1 mutants, chains of meiotic germ nuclei spanned the rachis and were partially enveloped by invaginations of germ line plasma membrane, similar to nuclei at the cortex. Extensions of the somatic sheath cells that surround the germ line protruded deep inside the rachis and were associated with displaced nuclei in fli-1 mutants. fli-1 encodes a molecule with leucine-rich repeats and gelsolin repeats similar to Drosophila flightless 1 and human Fliih, which have been shown to act as cytoplasmic actin regulators as well as nuclear transcriptional regulators. Mutations in let-60 Ras, previously implicated in germ line development, were found to cause the Glm phenotype. Constitutively-active LET-60 partially rescued the fli-1 Glm phenotype, suggesting that LET-60 Ras and FLI-1 might act together to control germ line morphogenesis. FLI-1 controls germ line morphogenesis and rachis organization, a process about which little is known at the molecular level. The LET-60 Ras GTPase might act with FLI-1 to control germ line morphogenesis.