Oocyte-specific gene Oog1 suppresses the expression of spermatogenesis-specific genes in oocytes
Oocyte-specific gene Oog1 suppresses the expression of spermatogenesis-specific genes in oocytes
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DOI：
10.1262/jrd.2018-024
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发表时间：
2018-08-01
影响因子：
1.8
通讯作者：
Minami, Naojiro
中科院分区：
文献类型：
--
作者：
Honda, Shinnosuke;Miki, Yuka;Minami, Naojiro
Oog 1是一种卵母细胞特异性基因，编码425个氨基酸的蛋白质，在小鼠4号和12号染色体上以5个拷贝存在。在小鼠卵母细胞中，Oog 1 mRNA的表达开始于胚胎的第15.5天，并几乎消失的晚期两细胞阶段。同时，OOG 1蛋白在卵巢囊肿的卵母细胞中可检测到，并在四细胞期消失;该蛋白在一细胞晚期至两细胞早期胚胎中被转运到细胞核。在这项研究中，我们研究了Oog 1在小鼠卵子发生过程中的作用。通过表达双链发夹Oog 1 RNA产生Oog 1 RNAi转基因小鼠，所述双链发夹Oog 1 RNA被加工成靶向Oog 1 mRNA的siRNA。定量RT-PCR结果显示，Oog 1基因敲除小鼠卵母细胞中Oog 1 mRNA的表达量显著降低，而精子发生相关转录物（Klhl 10，Tekt 2，Tdrd 6和Tnp 2）的丰度在Oog 1基因敲除小鼠卵巢中增加。Tdrd 6参与拟染色体的形成，Tnp 2有助于精子头部的形成，Tekt 2是纤毛和鞭毛微管形成所必需的，而KIhl 10在细长精子分化中起关键作用。这些结果表明，Oog 1下调女性生殖细胞中精子发生相关基因的表达，使其能够正常发育成卵母细胞。
Oog1, an oocyte-specific gene that encodes a protein of 425 amino acids, is present in five copies on mouse chromosomes 4 and 12. In mouse oocytes, Oog1 mRNA expression begins at embryonic day 15.5 and almost disappears by the late two-cell stage. Meanwhile, OOG1 protein is detectable in oocytes in ovarian cysts and disappears by the four-cell stage; the protein is transported to the nucleus in late one-cell to early two-cell stage embryos. In this study, we examined the role of Oog1 during oogenesis in mice. Oog1 RNAi-transgenic mice were generated by expressing double-stranded hairpin Oog1 RNA, which is processed into siRNAs targeting Oog1 mRNA. Quantitative RT-PCR revealed that the amount of Oog1 mRNA was dramatically reduced in oocytes obtained from Oog1-knockdown mice, whereas the abundance of spermatogenesis-associated transcripts (Klhl10, Tekt2, Tdrd6, and Tnp2) was increased in Oog1 knockdown ovaries. Tdrd6 is involved in the formation of the chromatoid body, Tnp2 contributes to the formation of sperm heads, Tekt2 is required for the formation of ciliary and flagellar microtubules, and KIhl10 plays a key role in the elongated sperm differentiation. These results indicate that Oog1 down-regulates the expression of spermatogenesis-associated genes in female germ cells, allowing them to develop normally into oocytes.