Characterization of allergens from urine and pelts of laboratory mice.
Characterization of allergens from urine and pelts of laboratory mice.
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实验室小鼠尿液和毛皮中过敏原的表征。
DOI：
10.1016/0161-5890(80)90105-4
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发表时间：
1980
影响因子：
3.6
通讯作者：
Michael J. Schumacher
中科院分区：
文献类型：
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作者：
Michael J. Schumacher
对CBA/H、BALB/c和C57BL小鼠尿液和CBA/H小鼠皮毛抗原进行了研究，以确定能使实验室工作人员对小鼠致敏的过敏原。在主要尿蛋白(MUP)‡复合体中发现了小鼠尿液中的有效过敏原，该复合体的分子量为16,500道尔顿(未还原)和22,000道尔顿(还原)。主要成分的等电点为4.9。CBA/H、BALB/c和C57BL尿中也含有低分子物质(15,000道尔顿)，其效力仅略低于MUP变应原，但含量相对较少。在这三个小鼠品系中，与IgE抗体结合的纯化MUP蛋白相互之间以及与小鼠房间灰尘中的过敏原发生广泛的交叉反应。在同一等电点上，等电点为4.9的尿蛋白与CBA/H皮试抗原的低分子组份之间存在完全的抗原交叉反应，提示皮试材料的部分致敏性可能与其MUP复合体成分的含量有关。然而，通过凝胶过滤层析和CBA/H毛皮材料的制备等电聚焦，鉴定出其他较高分子量(62,000道尔顿)和等电点在5.5至6.7之间的过敏原。
Antigens from CBA/H, BALB/c and C57BL mouse urine and CBA/H mouse pelts were studied to identify allergens that sensitize laboratory workers exposed to mice. Potent allergens in mouse urine were identified within the major urinary protein (MUP)‡ complex, which was found to have a molecular weight of 16,500 daltons (unreduced) and 22,000 daltons (reduced). The isoelectric point of the major component was 4.9. CBA/H, BALB/c and C57BL urine also contained material of low molecular weight (< 15,000 daltons) which was only slightly less potent than MUP allergens, but was present in relatively small amounts. In the three mouse strains, purified MUP proteins that bound to IgE antibodies cross-reacted extensively with one another, and with allergens in dust from a mouse room. There was complete antigenic cross-reactivity between urinary protein of pI 4.9 and low molecular weight fractions of CBA/H pelt antigens at the same isoelectnc point, suggesting that part of the allergenicity of pelt material may result from its content of components of the MUP complex. However, other allergens with a higher molecular weight (62,000 daltons) and isoelectric points between 5.5 and 6.7 were identified by gel filtration chromatography and preparative isoelectric focusing of CBA/H pelt material.