Capturing chromosome conformation
Capturing chromosome conformation
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DOI：
10.1126/science.1067799
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发表时间：
2002-02-15
期刊：
影响因子：
56.9
通讯作者：
Kleckner, N
中科院分区：
文献类型：
--
作者：
Dekker, J;Rippe, K;Kleckner, N
我们描述了一种检测任意两个基因组位点之间相互作用频率的方法。生成同一染色体或不同染色体上位点之间相互作用频率的矩阵，可揭示它们的相对空间位置，并提供有关染色质纤维物理特性的信息。这种方法可应用于从细菌到人类的生物体中整个基因组的空间组织研究。利用酿酒酵母，我们能够确认细胞核内染色体组织已知的定性特征以及减数分裂过程中该组织的动态变化。我们还在细胞周期的G₁期分析了酵母三号染色体。我们发现染色质始终具有高度的柔韧性。此外，发现功能不同的富含AT和富含GC的区域呈现出不同的构象，并且可以确定三号染色体的群体平均三维模型。三号染色体呈现为一个扭曲的环。
We describe an approach to detect the frequency of interaction between any two genomic loci. Generation of a matrix of interaction frequencies between sites on the same or different chromosomes reveals their relative spatial disposition and provides information about the physical properties of the chromatin fiber. This methodology can be applied to the spatial organization of entire genomes in organisms from bacteria to human. Using the yeast Saccharomyces cerevisiae, we could confirm known qualitative features of chromosome organization within the nucleus and dynamic changes in that organization during meiosis. We also analyzed yeast chromosome III at the G(1) stage of the cell cycle. We found that chromatin is highly flexible throughout. Furthermore, functionally distinct AT- and GC-rich domains were found to exhibit different conformations, and a population-average 3D model of chromosome III could be determined. Chromosome III emerges as a contorted ring.