Response of epithelial cells infected by Treponema denticola
Response of epithelial cells infected by Treponema denticola
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齿垢密螺旋体感染上皮细胞的反应
DOI：
10.1111/odi.12794
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Ishihara K.
中科院分区：
文献类型：
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作者：
Kokubu E;Inoue T;Ishihara K.
在龈沟中，上皮细胞与牙周致病菌之间的相互作用对牙周炎的发生发展至关重要，齿垢密螺旋体（Treponema denticolas）是慢性牙周炎的主要病原体，具有多种毒力因子，如主要表面蛋白（major surface protein，Msp）和脯氨酰苯丙氨酸特异性蛋白酶（prolyl-phenylalanine-specific protease，dentisin）。在这里，我们研究了用齿垢锥虫感染的上皮细胞的行为，测量白细胞介素（IL）-1 β、IL-6、β防御素2（BD-2）和热休克蛋白70（HSP 70）的表达。结果感染齿突毛癣菌野生型和突变株后，IL-6和HSP 70的表达水平均明显升高。拜特. denticola野生型菌株在24小时诱导的BD-2水平显著高于牙本质素缺陷型突变体。IL-1β mRNA水平在野生型菌株和牙本质素缺陷型菌株中略低于未感染对照组。结论这些结果表明，BD-2水平受Msp和牙本质素的影响。这种作用可能有助于破坏上皮细胞对根除齿垢毛癣菌的反应。
ObjectiveIn the gingival crevice, the interaction between epithelial cells and periodontopathic bacteria is important for the development of periodontitis.Treponema denticolais a major pathogen of chronic periodontitis and possesses several virulence factors, such as major surface protein (Msp) and prolyl‐phenylalanine‐specific protease (dentilisin). Here, we investigated the behaviours of epithelial cells infected withT. denticolaby measuring the expression of interleukin (IL)‐1β, IL‐6, β defensin 2 (BD‐2) and heat‐shock protein 70 (HSP70).MethodsEpithelial cells were infected withT. denticolawild‐type strain, Msp‐deficient mutant or dentilisin‐deficient mutant, and the expression levels of the above targets were analysed by polymerase chain reaction.ResultsInfection withT. denticolawild‐type strain and mutants induced the production of IL‐6 and HSP70. The level of BD‐2 induced byT. denticolawild‐type strain at 24 hr was significantly higher than that of the dentilisin‐deficient mutant. The level ofIL‐1β mRNA in the wild‐type strain and dentilisin‐deficient mutant was slightly lower than that in the uninfected control.ConclusionThese results suggest that the levels of BD‐2 were affected by Msp and dentilisin. This effect may contribute to the disruption of the response of epithelial cells to eradicateT. denticola.