Dielectrophoretic fluidic cell fractionation system
Dielectrophoretic fluidic cell fractionation system
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DOI：
10.1016/j.aca.2003.08.071
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发表时间：
2004-04-01
影响因子：
6.2
通讯作者：
Kaler, KVIS
中科院分区：
文献类型：
--
作者：
Li, YL;Kaler, KVIS
本文介绍了一种能够将悬浮液中的完整生物细胞分离成更纯的亚群的DEP流体系统的开发和实验验证。这是通过采用特殊形状的非均匀电场来实现的，该电场由安装在绝缘玻璃基板上的微型制造平面微电极阵列合成。为了提高细胞分选的效率，微电极被一个可变频率的交流电(AC)电压源单独偏置，这使得我们可以利用正和负介电泳法(DEP)来影响细胞分离。此外，通过适当建立由鞘流支持的细胞流，以连续的方式实现这种分级。建议的DEP流体分馏可配置为在三(3)种不同模式下运行。然而，在这部作品中，只对一种操作模式进行了详细的说明。电场和力分布的模拟，以及在模型细胞(植物原生质体)上获得的实验结果，证实了我们的理论预测，并进一步证明了在较宽的频率范围内(10 Hz到5 kHz)分离效率和吞吐量都有所改善。(C)2003爱思唯尔BX保留所有权利。
This paper presents the development and experimental verification of a DEP fluidic system capable of fractionation of intact biological cells in suspension into purer subpopulations. This was accomplished by employing a specially shaped nonuniform electric field, synthesized by microfabricated planar microelectrode arrays, housed on an insulating glass substrate. To improve the efficiency of cell sorting, the microelectrodes are individually biased by a variable frequency alternating current (ac) voltage source, which allows us to exploit both positive and negative dielectrophoresis (DEP) to affect cell separation. Furthermore, through suitable establishment of a cell stream supported by sheath flow, such fractionation is achieved in a continuous fashion. The proposed DEP fluidic fractionation may be configured to operate in three (3) different modes. In this work, however, a detailed account is only presented for one mode of operation. The simulation of the electric field and force profiles, together with the experimental results obtained on model cells (plant protoplasts), confirm our theoretical predictions and furthermore demonstrate improvements in both separation efficiency and throughput over a wide range of frequencies (10 Hz to 5 kHz). (C) 2003 Elsevier BX All rights reserved.