Chemical probes and tandem mass spectrometry: a strategy for the quantitative analysis of proteomes and subproteomes.
Chemical probes and tandem mass spectrometry: a strategy for the quantitative analysis of proteomes and subproteomes.
复制标题
DOI：
10.1016/j.cbpa.2003.12.001
复制
发表时间：
2004-02
影响因子：
7.8
通讯作者：
Hui Zhang;Wei Yan;R. Aebersold
中科院分区：
文献类型：
--
作者：
Hui Zhang;Wei Yan;R. Aebersold
使用质谱和稳定同位素稀释的定量蛋白质组分析正广泛应用于生物系统的功能分析以及用于检测临床、诊断或预后标志物蛋白。由于蛋白质组的巨大复杂性，在不久的将来不太可能实现对它们的全面分析。然而，近年来，定量蛋白质组学分析已取得重大进展，重点关注富含生物学或临床重要信息的特定蛋白质类别或亚蛋白质组。这些项目通常联合收割机使用化学探针，这些探针对目标蛋白质组具有特异性，可能含有稳定的同位素特征，用于准确定量，并结合使用自动串联质谱和生物信息学工具进行数据分析。在这篇综述中，我们总结了基于串联质谱和化学探针的定量亚蛋白质组分析的技术和概念进展。
Quantitative proteome profiling using mass spectrometry and stable isotope dilution is being widely applied for the functional analysis of biological systems and for the detection of clinical, diagnostic or prognostic marker proteins. Because of the enormous complexity of proteomes, their comprehensive analysis is unlikely to be routinely achieved in the near future. However, in recent years, significant progress has been achieved focusing quantitative proteomic analyses on specific protein classes or subproteomes that are rich in biologically or clinically important information. Such projects typically combine the use of chemical probes that are specific for a targeted group of proteins and may contain stable isotope signatures for accurate quantification with automated tandem mass spectrometry and bioinformatics tools for data analysis. In this review, we summarize technical and conceptual advances in quantitative subproteome profiling based on tandem mass spectrometry and chemical probes.